中文摘要：本研究旨在评估用赭曲霉毒素A（OTA）污染的饲料饲喂鸡，在有和没有益生菌制剂时，其便粪水中的基因毒性和细胞毒性对比情况。研究结果表明，用含有益生菌制剂的饲料补充喂养后，其基因毒性和细胞毒性明显降低。
外文摘要：The aim of the present study was to assess the genotoxicity and cytotoxicity of the faecal water of chickens fed ochratoxin A (OTA) contaminated feed with and without probiotic preparation. The study was performed on 20 healthy female Ross broiler chickens divided into 4 groups: control chickens - fed with non-supplemented feed; PP chickens - fed feed supplemented with the probiotic preparation; OTA chickens - fed feed contaminated with 1 mg per kg of OTA; OTA + PP chickens - fed feed contaminated with 1 mg per kg of OTA and supplemented with the probiotic preparation. Faecal water samples were collected on the 35(th) day of life of chickens from each group. Genotoxicity was measured using the comet assay, and cytotoxicity by means of MTT tests. Mean DNA damage, measured as the percentage of DNA in the tails of the comets, was 8.50 ± 1.10 for chickens fed OTA at 1 mg/kg and 6.41 ± 0.67 in the controls. The supplementation of feed with the probiotic preparation decreased the extent of DNA damage to 4.74 ± 0.78. In the control group of chickens the average cytotoxicity was 38.5 ± 0.5 (in MTT), while in the probiotic preparation group (PP group) it was 31.8 ± 0.7 (in MTT). After supplementation of the feed with the probiotic preparation, the genotoxicity and cytotoxicity were decreased in a statistically significant manner.
外文关键词：ochratoxin A；probiotics；chicken；genotoxicity；cytotoxicity
作者：Slizewska, Katarzyna; Nowak, Adriana; Smulikowska, Stefania
作者单位：Lodz Univ Technol,Poland.
期刊名称：ACTA BIOCHIMICA POLONICA
期刊影响因子：1.187
出版年份：2016
出版刊次：11
点击下载：鸡食用了赭曲霉毒素A污染的饲料后，用益生菌制剂可以降低其粪便水中的基因毒性和细胞毒性