中文摘要：已有报导表明，三磷酸腺苷（ATP）-结合盒（ABC）转运蛋白的多药耐药抗性（PDR）亚家族参与了植物的不同生物过程。本研究中，在抗小麦赤霉病（FHB）的小麦品种宁7840中克隆了三个新的PDR基因，它们分别位于小麦染色体6A，6B和6D，以此来分析与小麦赤霉病抗性有关的小麦多药耐药基因功能。本研究发现，TaPDR7的这三个基因受水杨酸（SA），茉莉酸甲酯（MeJA）和脱落酸（ABA）三种激素的负调控，而受吲哚乙酸酸（IAA）的正向调控。
外文摘要：The pleiotropic drug resistance (PDR) sub-family of adenosine triphosphate (ATP)-binding cassette (ABC) transporter had been reported to participate in diverse biological processes of plant. In this study, we cloned three novel PDR genes in Fusarium head blight (FHB) resistant wheat cultivar Ning 7840, which were located on wheat chromosomes 6A, 6B and 6D. In phylogeny, these genes were members of cluster I together with AePDR7 and BdPDR7. Subcellular localization analysis showed that TaPDR7 was expressed on the plasmalemma. The quantitative real time PCR (RT-PCR) analysis showed that this gene and its probable orthologues in chromosomes 6B and 6D were both up-regulated sharply at 48 h after infected by Fusarium graminearum and trichothecene deoxynivalenol (DON) in spike. When knocking down the transcripts of all TaPDR7 members by barely stripe mosaic virus-induced gene silencing (BSMV-VIGS) system, it could promote the F. graminearum hyphae growth and made larger pathogen inoculation points in Ning 7840, which suggested that TaPDR7 might play an important role in response to F. graminearum. Although salicylic acid (SA), methyl jasmonate (MeJA) and abscisic acid (ABA) had been reported to possibly regulate wheat FHB resistance, here, we found that the three members of TaPDR7 were negatively regulated by these three hormones but positively regulated by indoleacetic acid (IAA).
外文关键词：PDR；FHB；VIGS
作者：Wang Gui-ping; Hou Wen-qian; Zhang Lei; Wu Hong-yan; Zhao Lan-fei; Du Xu-ye; Ma Xin; Li An-fei; Wang Hong-wei; Kong Ling-rang
作者单位：山东农业大学
期刊名称：JOURNAL OF INTEGRATIVE AGRICUL
期刊影响因子：0.724
出版年份：2016
出版刊次：11
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