研制基于氧化铋纳米棒的免疫传感器,用于真菌毒素检测

Bismuth oxide nanorods based immunosensor for mycotoxin detection

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中文摘要:作者将氧化铋纳米棒 (nBi2O3)电泳沉积到镀铟锡氧化物(ITO)膜的玻璃基板上,研制了一种有效的免疫传感器。这种免疫传感器可以通过固定抗黄曲霉毒素单克隆抗体 (Ab-AFB1)和牛血清白蛋白(BSA)来检测黄曲霉毒素B1。
外文摘要:We report results of the studies relating to fabrication of an efficient immunosensor based on bismuth oxide nanorods (nBi2O3), electrophoretically deposited onto indium-tin-oxide (ITO) coated glass substrate. This immunosensor was fabricated by immobilization of anti-aflatoxin monoclonal antibodies (Ab-AFB1) and bovine serum albumin (BSA) for aflatoxin B1 detection. The structural and morphological studies of n-Bi2O3 have been carried out by XRD, UV–vis spectrophotometer; SEM, AFM and FTIR. It was found that the nBi2O3 provided improved sensing characteristics to the electrode interface in terms of electroactive surface area, diffusion coefficient, charge transfer rate constant and electron transfer kinetics. The results of electrochemical response studies of this BSA/Ab-AFB1/nBi2O3/ITO immunosensor revealed good linearity in the range of 1–70 ng dL− 1 with low detection limit of 8.715 ng/dL, improved sensitivity of 1.132 μA/(ng/dL cm− 2), regression coefficient R2 of 0.918 and reproducibility of > 11 times. The association constant for the BSA/Ab-AFB1/nBi2O3/ITO immunosensor was determined as 7.318 ng/dL.
外文关键词:Bismuth oxide nanorods (nBi(2)O(3));Aflatoxin B1;Immunosensor;Electrochemical studies Natl Phys Lab;India.
作者:Solanki, Pratima R.; Singh, Jay; Rupavali, Bharti; Tiwari, Sachchidanand; Malhotra, Bansi D.
作者单位:Natl Phys Lab;India.
期刊名称:MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
期刊影响因子:3.42
出版年份:2017
出版刊次:1
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  1. 编译服务:农产品质量安全
  2. 编译者:任妮
  3. 编译时间:2016-12-26