中文摘要：尽管对转基因作物有很多争议，但转基因作物的种植仍在不断增加，且根据欧盟法律的规定，转基因作物含量高于0.9%的食品需加标签。因而，迫切需要能够用于快速和有效检测食品中转基因生物含量的方法。本文研发了一种在MON810转基因玉米中有效检测Cry1Ab蛋白含量的电化学免疫传感器。作者通过循环伏安法和电化学阻抗谱模式直接监控和测量了商用Cry1Ab蛋白与多克隆抗体（PAb）的相互作用。研究发现，该电化学免疫传感器可实现对从不同转基因玉米质量百分比（0%, 0.5%, 1%, 2.5% and 5%）的玉米种子中提取的Cry1Ab蛋白的简单和快速检测。另外，作者用酶联免疫吸附试验方法（ELISA）对Cry1Ab蛋白的检测结果进行了交叉检验。研究结果表明，该电化学免疫传感器可作为查证欧盟法律遵从性的工具，来检测转基因玉米中转基因蛋白Cry1Ab的含量。
外文摘要：Regardless the controversies surrounding genetically modified organisms (GMO), their cultivation is constantly increasing and in according to the EU legislation, labeling is mandatory for products containing EU-authorized-GMO higher than 0.9%. Thereby, new analytical strategies for rapid and effective detection of GMO on foodstuffs are required. In this work, an electrochemical immunosensor for effective determination of Cry1Ab protein from MON810 transgenic maize (EU-authorized-GMO) is described. The immunosensor was developed onto indium tin oxide (ITO) electrodes modified by 3-aminopropyltrimethoxysilane (APTES) monolayer to covalently immobilize Anti-Cry1Ab polyclonal antibodies. The protein interaction with the polyclonal antibody (PAb) recognition platform was directly monitored and measured by cyclic voltammetry and electrochemical impedance spectroscopy using commercially Cry1Ab protein. After the analytical features optimization a linear response from 1 to 10 ng mL(-1), a limit of detection (LOD) of 0.37 ng mL(-1) and a limit of quantification (LOQ) of 1.23 ng mL(-1) - which provided accurate results (RSD <7.5%) - were achieved. The immunosensor allowed a simple and fast detection of Cry1Ab protein extracted from maize seeds with different GM maize mass percentages (0%, 0.5%, 1%, 2.5% and 5%). To crosscheck the detection of Cry1Ab protein, an enzyme-linked immunosorbent assay (ELISA) was used. The results indicate that the immunosensor is suitable for the transgenic protein Cry1Ab detection in GM maize representing a successfully tool to verify the compliance of the EU regulations.
外文关键词：Electrochemical immunosensor；Cry1Ab；Electrochemical impedance spectroscopy；Genetically modified maize
作者：Freitas, Maria；Correr, Wagner；Cancino-Bernardi, Juliana；等
作者单位：圣保罗大学
期刊名称：SENSORS AND ACTUATORS B-CHEMICAL
期刊影响因子：4.758
出版年份：2016
出版刊次：12
点击下载：利用阻抗免疫传感器检测转基因玉米种子中Cry1Ab蛋白的含量