中文摘要：本文的研究目的是对TT51-1进行食品安全评价，TT51-1是一种表达Cry1Ab/Ac蛋白（Bt毒素）的转基因水稻，且对大多数鳞翅目害虫具有高抗性。本文将十六只雄性和16只雌性食蟹猴随机分为四组：常规稻、阳性对照、17.5%的转基因水稻和70%的转基因水稻。在非转基因水稻、阳性对照和转基因水稻组分别对食蟹猴饲喂含70%非转基因水稻的饲料、含17.5%的转基因水稻和含70%的转基因水稻17.5%。研究发现，六个月的喂养中没有出现异常观察值；喂养非转基因水稻组和喂养转基因水稻组的食蟹猴，在体重、摄食量、心电图、血液学、外周血淋巴细胞的免疫表型、丝裂原诱导的外周血淋巴细胞增殖、脾淋巴细胞增殖、KLH-T细胞依赖性抗体反应、器官重量和比值和组织学表现等参数上均无显著差异；相对后者，喂养非转基因水稻组食蟹猴的体温、血清丙氨酸氨基转移酶（ALT）水平明显较高，而其血清中K+、Cl-的浓度和细胞因子较低。另外由于在这项研究中没有观察到剂量或时间性的变化，且食蟹猴的组织学表现正常，因而上述差异不是由转基因水稻的喂养引起的。为期6个月的TT51-1喂养研究显示，TT51-1对食蟹猴没有产生不良的免疫毒理效应。
外文摘要：The present study was performed to evaluate the food safety of TT51-1, a new type of genetically modified rice that expresses the Cry1Ab/Ac protein (Bt toxin) and is highly resistant to most lepidopteran pests. Sixteen male and 16 female cynomolgus monkeys were randomly divided into four groups: conventional rice (non-genetically modified rice, non-GM rice), positive control, 17.5% genetically modified rice (GM rice) and 70% GM rice. Monkeys in the non-GM rice, positive control, and GM rice groups were fed on diets containing 70% non-GM rice, 17.5% GM rice or 70% GM rice, respectively, for 182 days, whereas animals in the positive group were intravenously injected with cyclophosphamide every other day for a total of four injections before the last treatment. Six months of treatment did not yield abnormal observations. Specifically, the following parameters did not significantly differ between the non-GM rice group and GM rice groups: body weight, food consumption, electrocardiogram, hematology, immuno-phenotyping of lymphocytes in the peripheral blood, mitogen-induced peripheral blood lymphocyte proliferation, splenocyte proliferation, KLH-T cell-dependent antibody response, organ weights and ratios, and histological appearance (p>0.05). Animals from the GM rice group differed from animals in the non-GM rice group (p<0.05) in several parameters: specifically, their body temperatures and serum alanine aminotransferase (ALT) levels were higher, whereas their levels of serum K+, Cl- and cytokines (IL-2, IL-4 and IL-5) were lower. Because dose- or time-dependent changes were not observed in this study and animals appeared histologically normal, the aforementioned differences were not considered to be adverse or related to the treatment with GM rice. In conclusion, a 6-month feeding study of TT51-1 did not show adverse immunotoxicological effects on cynomolgus monkeys.
作者：Tan, Xiaoyan；Zhou, Xiaobing；Tang, Yao；等
作者单位：Natl Ctr Safety Evaluat Drugs
期刊名称：PLOS ONE
期刊影响因子：3.057
出版年份：2016
出版刊次：9
点击下载：通过为期6个月的食蟹猴喂养试验来对表达Cry1Ab/Ac蛋白的转基因水稻（TT51-1）进行免疫毒理学评价