中文摘要：内生微生物的多样性可能会因寄主植物的基因型和物候期发生变化。本文评估了玉米的物候期、转基因和基因组成对内生细菌和真菌群落的影响。玉米群体由本地玉米RS和三种同基因杂交玉米组成；其中一种同基因杂交玉米（NGM）不是转基因作物，另一种同基因杂交玉米（HX）含有转基因Cry1F和pat，Cry1F和pat可分别对鳞翅目昆虫和草铵膦除草剂产生抗性；最后一种同基因杂交玉米（Hxrr）含有转基因CP4 EPSPS、Cry1F、帕特，除了Hx具有的特性外，Hxrr可对农达除草剂产生抗性。本文利用PCR-DGGE分析四个物候期内细菌和真菌的特异性引物来进行叶组织的评价。研究发现，内生细菌只在物候期聚集；真菌群落的结构是由各物候期的玉米基因型集成。在四个评价阶段，本地玉米RS与三个杂交种（NGM，Hx和hxrr）的真菌群落不同。在生殖期，两种转基因杂交玉米（Hx和Hxrr）的真菌群落有所不同，且Hxrr与NGM的真菌群落不同。研究结构表明，玉米群体的遗传组成（尤其是转基因）是在检测玉米叶片中内生真菌群落变化的决定因素。
外文摘要：The diversity of endophytic microorganisms may change due to the genotype of the host plant and its phenological stage. In this study we evaluated the effect of phenological stage, transgenes and genetic composition of maize on endophytic bacterial and fungal communities. The maize populations were composed of a local variety named Rosado ( RS) and three isogenic hybrids. One isogenic hybrid was not genetically modified ( NGM). Another hybrid ( Hx) contained the transgenes cry1F and pat ( T1507 event), which provide resistance to insects of the order Lepidoptera and tolerance to the glufosinate-ammonium herbicide, respectively. The third hybrid ( Hxrr) contained the transgene cp4 epsps ( NK603 event) combined with the transgenes cry1F and pat ( T1507 event), which allow tolerance to the Roundup Ready herbicide, besides the characteristics of Hx. Evaluation of the foliar tissue was done through PCR-DGGE analysis, with specific primers for bacteria and fungi within four phenological stages of maize. The endophytic bacteria were only clustered by phenological stages; the structure of the fungal community was clustered by maize genotypes in each phenological stage. The fungal community from the local variety RS was different from the three hybrids ( NGM, Hx and Hxrr) within the four evaluated stages. In the reproductive stage, the fungal community from the two transgenic hybrids ( Hx and Hxrr) were separated, and the Hxrr was different from NGM, in the two field experiments. This research study showed that the genetic composition of the maize populations, especially the presence of transgenes, is the determining factor for the changes detected in the endophytic fungal community of maize leaves.
外文关键词：PCR-DGGE；Plant genetic resource；rDNA 16S/18S；Zea mays L；Bacteria；Fungi
作者：da Silva, Kelly Justin；de Armas, Rafael Dutra；Soares, Claudio Roberto F. S；等
作者单位：Univ Fed Santa Catarina
期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
期刊影响因子：1.532
出版年份：2016
出版刊次：11
点击下载：本地玉米、转基因玉米和常规同基因杂交玉米在不同生长阶段的内生微生物群落变化