中文摘要：脱氧雪腐镰刀菌烯醇(DON)和雪腐镰刀菌烯醇(NIV)） 是全球分布最广的污染小粒谷类作物的单端孢霉烯族毒素。本研究用原位富集法从土壤中分离到一个细菌菌群PGC-3，具有脱环氧化活性。它能通过生物转化，将DON转化成脱环氧DON，NIV转化成脱环氧NIV。在有氧条件下，PGC-3能在很大的PH值（5-10）及温度（20-37 °C）范围内展现其脱环氧活性。本研究结果为进一步开发谷类单端孢霉烯族毒素解毒生物活性剂奠定了基础，并能用于研究解毒基因及其调控机理。
外文摘要：Globally, the trichothecene mycotoxins deoxynivalenol (DON) and nivalenol (NIV) are among the most widely distributed mycotoxins that contaminate small grain cereals. In this study, a bacterial consortium, PGC-3, with de-epoxydation activity was isolated from soil by an in situ soil enrichment method. Screening of 14 soil samples that were sprayed with DON revealed that 4 samples were able to biotransform DON into de-epoxydized DON (dE-DON). Among these, the PGC-3 consortium showed the highest and most stable activity to biotransform DON into dE-DON and NIV into dE-NIV. PGC-3 exhibited de-epoxydation activity at a wide range of pH (5-10) and temperatures (20-37 °C) values under aerobic conditions. Sequential subculturing with a continued exposure to DON substantially reduced the microbial population diversity of this consortium. Analyses of the 16S rDNA sequences indicated that PGC-3 comprised 10 bacterial genera. Among these, one species, Desulfitobacterium, showed a steady increase in relative abundance, from 0.03% to 1.55% (a 52-fold increase), as higher concentrations of DON were used in the subculture media, from 0 to 500 μg/mL. This study establishes the foundation to further develop bioactive agents that can detoxify trichothecene mycotoxins in cereals and enables for the characterization of detoxifying genes and their regulation. 
外文关键词：trichothecenes；deoxynivalenol；aerobic de-epoxydation；soil bacterium；16S rDNAsequencing
作者：He, WJ；Yuan, QS；Zhang, YB；Guo, MW；Gong, AD；Zhang, JB；Wu, AB；Huang, T；Qu, B；Li, HP；Liao, YC
作者单位：Huazhong Agr Univ
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2016
出版刊次：10
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