中文摘要：高分辨率质谱法一直是实验室食品农药残留检测的常用方法，最新的一些仪器设备更新也使得简单的色谱分析可监测到的活性化合物及其转化产物的数量大大增加。因此，迫切需要一个具有非特异性、通用且能对物理化学特性不同的化合物进行高效提取的样本处理过程。而QuEChERS法及其改进方法、“dilute-and-shoot”提取方法与高分辨率质谱色谱法联用在食品农药残留检测方面具有巨大的应用潜力。本文对上述组合方法对复杂基质检测的灵敏度和特异性、适用范围、提取效率、电离、基质效应等进行了研究，并通过基质匹配校准和同位素标记标准对清洗、稀释和基质补偿等步骤进行了改进。
外文摘要：The use of high-resolution MS (HRMS) is becoming popular in laboratories for the determination of pesticide residues in food commodities. The recent improvements in the instrumentation have helped to increase the number of active compounds and transformation products that can be monitored within a simple chromatographic run. However, prior to instrumental determination, it is necessary to perform a nonspecific, or generic, sample treatment that allows the efficient extraction of several compounds with very relevant differences in their physical and chemical properties. In this sense, the quick, easy, cheap, effective, rugged, and safe (QuEChERS) method and its several modifications and the "dilute-and-shoot" extraction methodology have already revealed an enormous potential for their use together with chromatographic techniques coupled to HRMS. The potentiality and limitations of such a methodological combination have been evaluated in terms of sensitivity and selectivity when they are applied to the analysis of complex food matrixes. An evaluation of the scope of the methods, in terms of efficiency of the extraction and ionization steps, as well as the matrix effect, has also been carried out. Different solutions for the matrix effect have been considered, including improvement in clean-up steps, sample dilution, and matrix compensation by matrix-matched calibration or by the use of isotopically labeled standards.
 
外文关键词：TIME-OF-FLIGHT; PERFORMANCE LIQUID-CHROMATOGRAPHY; PRESSURE GAS-CHROMATOGRAPHY; PRODUCTS CAMELLIA-SINENSIS; QUADRUPOLE-TIME; TRIPLE QUADRUPOLE; NUTRACEUTICAL PRODUCTS; CHEMICAL-IONIZATION; VETERINARY DRUGS; DIETARY-SUPPLEMENTS
作者：Romero-Gonzalez, Roberto; Arrebola Liebanas, Francisco Javier; Lopez-Ruiz, Rosalia; Garrido Frenich, Antonia
作者单位：Univ Almeria, Analyt Chem Area, Dept Chem & Phys, Agrifood Campus Int Excellence,Res Ctr Agr & Food, Carretera Sacramento S-N, E-04120 Almeria, Spain.
期刊名称：JOURNAL OF AOAC INTERNATIONAL
期刊影响因子：0.918
出版年份：2016
出版刊次：11
点击下载：采用高分辨率质谱法进行食品农药残留检测的样本处理方法研究