中文摘要：饲料受霉菌毒素污染会带来严重的健康风险，因此很有必要建立一种用于净化霉菌毒素污染的实用方法。本研究调查了柠檬酸(CA)和乳酸(LA)对镰刀菌毒素污染的饲料中常见的单端孢霉烯族毒素的作用。结果显示用CA和LA处理降低了饲料中的DON浓度：不论时间长短，用5%LA溶液处理后饲料中的初始DON浓度降低了一半，并对15Ac-DON、15-羟基-倍半萜及接骨镰菌醇也有相似的效果；而雪腐镰刀菌烯醇浓度仅在用LA处理时降低。相反，CA和LA处理对包括玉米赤霉烯酮、伏马菌素和倍半萜在内的几种霉菌毒素及其衍生物的浓度没有或仅有很小的作用。总之，目前的研究表明，用5%的LA和CA溶液能降低常见的单端孢霉烯族毒素，特别是DON及其衍生物15Ac-DON的浓度。而总的毒性作用及潜在机制还需进一步深入研究。
外文摘要：Exposure to mycotoxin-contaminated feeds represents a serious health risk. This has necessitated the need for the establishment of practical methods for mycotoxin decontamination. This study investigated the effects of citric acid (CA) and lactic acid (LA) on common trichothecene mycotoxins in feeds contaminated with Fusarium mycotoxins. Contaminated feed samples were processed either with 5% CA or 5% LA solutions in a ratio of 1:1.2 (w/v) for 5, 24, or 48 h, and analyzed for multiple mycotoxin metabolites using a liquid chromatography-tandem mass spectrometric method. The analyses showed that treating the feed with CA and LA lowered the concentration of deoxynivalenol (DON), whereby 5% LA lowered the original DON concentration in the contaminated feed samples by half, irrespective of the processing time. Similar lowering effects were observed for the concentrations of 15Ac-DON, 5-hydroxyculmorin, and sambucinol. The concentration of nivalenol was only lowered by the LA treatment. In contrast, CA and LA treatments showed no or only small effects on the concentration of several mycotoxins and their derivatives, including zearalenone, fumonisins, and culmorin. In conclusion, the present results indicate that the use of 5% solutions of LA and CA might reduce the concentration of common trichothecene mycotoxins, especially DON and its derivate 15Ac-DON. However, further research is required to determine the effect on overall toxicity and to identify the underlying mechanisms.
外文关键词：citric acid； grain treatment； lactic acid； mycotoxin decontamination； trichothecenes
作者：Humer, E； Lucke, A； Harder, H； Metzler-Zebeli, BU； Bohm, J； Zebeli, Q
作者单位：Univ Vet Med Vienna
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2016
出版刊次：10
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