中文摘要：为了探讨转基因作物的非预期效应，本文采用双向凝胶电泳法和质谱分析法对转基因植酸酶玉米和非转基因植物的叶片进行了比较蛋白质组学分析。本研究成功鉴定了57个差异表达蛋白（DEPS），其中44种代表独特的蛋白质。鉴定蛋白的功能分类显示，这些DEPS主要参与碳水化合物的运输和代谢，其次参与翻译后修饰。KEGG通路分析表明，大部分的DEPS参与光合作用的碳固定。另外发现，有15种蛋白质代表蛋白质的相互作用，这些蛋白质主要参与糖酵解和碳固定。研究结果表明，尽管植酸酶转基因玉米和非转基因玉米叶片存在一些显著差异，但两者的蛋白质组模式没有显著差异；且没有一种DEPs被鉴定为新型毒蛋白或过敏蛋白。另外，叶片蛋白质组之间的差异可能是转基因和杂交的影响。
外文摘要：To investigate unintended effects in genetically modified crops (GMCs), a comparative proteomic analysis between the leaves of the phytase-transgenic maize and the non-transgenic plants was performed using two-dimensional gel electrophoresis and mass spectrometry. A total of 57 differentially expressed proteins (DEPs) were successfully identified, which represents 44 unique proteins. Functional classification of the identified proteins showed that these DEPs were predominantly involved in carbohydrate transport and metabolism category, followed by post-translational modification. KEGG pathway analysis revealed that most of the DEPs participated in carbon fixation in photosynthesis. Among them, 15 proteins were found to show protein-protein interactions with each other, and these proteins were mainly participated in glycolysis and carbon fixation. Comparison of the changes in the protein and tanscript levels of the identified proteins showed that most proteins had a similar pattern of changes between proteins and transcripts. Our results suggested that although some significant differences were observed, the proteomic patterns were not substantially different between the leaves of the phytase-transgenic maize and the non-transgenic isogenic type. Moreover, none of the DEPs was identified as a new toxic protein or an allergenic protein. The differences between the leaf proteome might be attributed to both genetic modification and hybrid influence.
外文关键词：biosafety assessment；comparative proteomics；genetically modified crop；phytase-transgenic maize；unintended effect
作者：Tan, Yanhua；Yi, Xiaoping；Wang, Limin；等
作者单位：海南大学
期刊名称：FRONTIERS IN PLANT SCIENCE
期刊影响因子：4.495
出版年份：2016
出版刊次：8
点击下载：转基因植酸酶玉米和非转基因玉米叶片的比较蛋白质组学分析
