表达mCry1Ac或maroacc基因的转基因玉米对蛋鸡生长、蛋质量、器官健康的影响

Effects of feeding transgenic corn with mCry1Ac or maroACC gene to laying hens for 12 weeks on growth, egg quality and organ health

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中文摘要:本研究的目的是探讨饲喂分别包含来自苏云金芽孢杆菌菌株的mCry1Ac基因的转基因玉米(BT-799)和包含来自农杆菌菌株的maroACC基因的转基因玉米(CC-2),对蛋鸡生长、蛋质量、器官健康指标的影响。mCry1Ac基因对玉米螟,maroACC基因对除草剂有抗性。健康母鸡被随机分配到四组,分别饲喂包含以下玉米配方的玉米豆粕日粮:非转基因近等基因玉米(控制组)、BT-799玉米、CC-玉米、市售的非转基因玉米(参照组)。首先对蛋鸡的性能(体重、采食量、产蛋量)和蛋质量进行测定。其次在12周的饲养结束后将其屠宰,记录其屠体产量和器官重量(心脏、肝、脾、肺、肾、胃和卵巢)的数据,并对其器官和肠道进行组织学分析。另外,用血清生化指标来对肝和肾的功能进行评估分析。研究发现,饲喂控制组、BT-799玉米组、CC-玉米组蛋鸡的体重、产蛋量和生产效率没有差异。研究结果表明, 饲喂含有BT-799玉米、CC-玉米饮食的蛋鸡与饲喂含有非转基因近等基因玉米饮食的蛋鸡,三者的性能、蛋质量、器官健康、屠体产量相似。
外文摘要:The objective of the present study was to investigate the effect of feeding two transgenic corn lines containing the mCry1Ac gene from Bacillus thuringiensis strain (BT-799) and the maroACC gene from Agrobacterium tumefaciens strain (CC-2), respectively, on growth, egg quality and organ health indicators. Expression of the mCry1Ac gene confers resistance to Pyrausta nubilalis and the maroACC gene confers tolerance to herbicides. Healthy hens (n=96 placed in cages; 3 hens/cage) were randomly assigned to one of four corn-soybean meal dietary treatments (8 cages/treatment) formulated with the following corn: non-transgenic near-isoline control corn (control), BT-799 corn, CC-2 corn and commercially available non-transgenic reference corn (reference). The experiment was divided into three 4-week phases (week 1 to 4, week 5 to 8 and week 9 to 12), during which hens were fed mash diets. Performance (BW, feed intake and egg production) and egg quality were determined. Following slaughter at the end of 12 weeks of feeding (n=8/treatment), carcass yield and organ weights (heart, liver, spleen, lung, kidneys, stomach and ovary) were recorded; organs and intestines were sampled for histological analysis. Analysis of serum biochemistry parameters to assess the liver and kidney function were performed. No differences in BW, egg production and production efficiency were observed between hens consuming the control diet and hens consuming the BT-799 or CC-2 diet. Haugh unit measures and egg component weights were similar between the control and test groups. Carcass yield was not affected by the diet treatment. Similar organosomatic indices and serum parameters did not indicate the characteristics of organ dysfunction. All observed values of the BT-799 and CC-2 groups were within the calculated tolerance intervals. This research indicates that the performance, egg quality, organ health and carcass yield of laying hens fed diets containing the BT-799 or CC-2 corn line were similar to that of laying hens fed diets formulated with the non-transgenic near-isoline corn with comparable genetic backgrounds.
外文关键词:maroACC;mCry1Ac;laying hen performance;egg quality;organ health
作者:Zhong, R. Q;Chen, L;Gao, L. X;等
作者单位:中国农业科学院
期刊名称:ANIMAL
期刊影响因子:1.508
出版年份:2016
出版刊次:8
  1. 编译服务:农产品质量安全
  2. 编译者:郭婷
  3. 编译时间:2017-01-06