中文摘要：本研究建立了一种快速、有效、环境友好的检测多种水果（梨、苹果、葡萄）中六种三唑类杀菌剂（三唑酮、三唑醇、氟环唑、氟硅唑、戊唑醇、和烯唑醇）的方法，该方法采用QuEChERS方法与离子液体分散液相微萃取法联用进行样本处理，然后采用高效液相色谱-二极管阵列进行残留检测。采用响应面法进行萃取条件优化，获得最佳萃取条件为：萃取剂体积63.7μl，分散剂体积0.43 mL，萃取时间1.7分钟。在最佳条件下，线性相关系数大于0.997， 0.2mg/kg、0.5mg/kg和1mg/kg三种加标水平下，回收率在63.8-119.1 %范围，相对标准差在1.1-12.6%之间，六种三唑类杀菌剂的检测限和定量限分别为3.4-26.8μg/kg和9.8-50.3μg/kg。该方法成功地应用于包括梨、苹果和葡萄等多种水果的农药痕量残留检测。
外文摘要：A rapid, efficient, and environmentally friendly method using quick, easy, cheap, effective, rugged, and safe (QuEChERS) combined with ionic liquid-based dispersive liquid-liquid microextraction (QuEChERS-IL-DLLME) prior to high-performance liquid chromatography coupled with photodiode array detection (HPLC-PDA) has been developed for the determination of six triazole fungicides (triazolone, triadimenol, epoxiconazole, flusilazole, tebuconazole, and diniconazole) in various fruits (pear, apple, and grapefruit). Several parameters affecting the extraction efficiency in IL-DLLME, such as type and volume of ionic liquid and acetonitrile volumes and extraction time, were investigated by single factor experiments. Then, the extractant volume, dispersant volume, and extraction time were optimized using response surface methodology (RSM). The optimal values were determined to be within an extractant volume of 63.7 mu L, a dispersant volume of 0.43 mL, and an extraction time of 1.7 min, respectively. Under the optimum conditions, an excellent linearity with determination coefficient higher than 0.997 was obtained. The average recoveries in three concentration levels (0.2, 0.5, and 1 mg kg(-1)) ranged from 63.8 to 119.1 %, respectively, and the relative standard deviations (RSDs) from 1.1 to 12.6 %. The limits of detection (LODs) (S/N = 3) and limits of quantification (LOQs) (S/N = 10) for the six triazole fungicides ranged from 3.4 to 26.8 mu g kg(-1) and 9.8 to 50.3 mu g kg(-1), respectively. The proposed method was successfully applied for the determination of trace amounts of triazole fungicides in various fruits including pear, apple, and grapefruit.
 
外文关键词：Ionic liquid (IL), Dispersive liquid-liquid microextraction (DLLME), QuEChERS, Triazole fungicides, Response surface methodology, Fruits
作者：Zhang, Yaohai; Zhang, Yan; Nie, Jiyun; Jiao, Bining; Zhao, Qiyang
作者单位：Chinese Acad Agr Sci, Inst Pomol, Lab Qual & Safety Risk Assessment Fruit, Minist Agr, Xingcheng 125199, Peoples R China.
期刊名称：Food Analytical Methods
期刊影响因子：2.167
出版年份：2016
出版刊次：9
点击下载：QuEChERS方法与离子液体分散液相微萃取法联用检测水果中三唑类杀菌剂残留并采用响应面法进行条件优化