中文摘要：本文深入研究了天然姜黄挥发油(EO)对黄曲霉的体外抗菌活性及其潜在机理和体内抗产黄曲霉毒素的性能。基于先前用气相色谱-质谱联用对姜黄EO进行的化学表征，发现它对菌丝生长、孢子萌发和黄曲霉毒素产生的抗菌活性呈剂量依赖关系。此外，抗菌效果还与真菌细胞内膜系统（包括质膜和线粒体）的破坏，特别是麦角甾醇合成、线粒体ATP酶、苹果酸脱氢酶和琥珀酸脱氢酶活性的抑制相关。另外，姜黄EO在黄曲霉毒素生物合成途径中，下调霉菌毒素基因的相关表达揭示了其抗产毒的机理。最后，对玉米中真菌污染的抑制效果表明姜黄EO具有成为环境友好的抗菌剂的潜力。
 
外文摘要：The antifungal activity and potential mechanisms in vitro as well as anti-aflatoxigenic efficiency in vivo of natural essential oil (EO) derived from turmeric (Curcuma longa L.) against Aspergillus flavus was intensively investigated. Based on the previous chemical characterization of turmeric EO by gas chromatography–mass spectrometry, the substantially antifungal activities of turmeric EO on the mycelial growth, spore germination and aflatoxin production were observed in a dose-dependent manner. Furthermore, these antifungal effects were related to the disruption of fungal cell endomembrane system including the plasma membrane and mitochondria, specifically i.e. the inhibition of ergosterol synthesis, mitochondrial ATPase, malate dehydrogenase, and succinate dehydrogenase activities. Moreover, the down-regulation profiles of turmeric EO on the relative expression of mycotoxin genes in aflatoxin biosynthetic pathway revealed its anti-aflatoxigenic mechanism. Finally, the suppression effect of fungal contamination in maize indicated that turmeric EO has potential as an eco-friendly antifungal agent.
外文关键词：Aspergillus flavus；Turmeric；Essential oil；Antifungal； Aflatoxin
作者：Hu, YC；Zhang, JM；Kong, WJ；Zhao, G；Yang, MH , Yichen；Zhang, Jinming；Kong, Weijun；Zhao, Gang；Yang, Meihua
作者单位：Chengdu Univ
期刊名称：FOOD CHEMISTRY
期刊影响因子：4.052
出版年份：2017
出版刊次：1
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