中文摘要：黄曲霉毒素由真菌产生，会造成食品污染，其中，黄曲霉毒素B1（AFB1）毒性最强，因此受到了极大的关注。本研究在荧光共振能量转移法的基础上，开发了纳米生物传感器，用来检测农产品中的AFB1。选择性试验表明纳米生物传感器是一种有前途的食品质量评价工具。该方法已经成功用于检测大米和花生中的AFB1。
外文摘要：Aflatoxins are potential food pollutants produced by fungi. Among them, Aflatoxin B1 (AFB1) is the most toxic. Therefore, a great deal of concern is associated with AFB1 toxicity. In this work, utilizing a FRET-based method, we have developed a nanobiosensor for detection of AFB1 in agricultural foods. Aptamer-conjugated Quantum dots (QDs) are adsorbed to Au nanoparticles (AuNPs) due to interaction of aptamers with AuNPs leading to quenching effect on QDs fluorescence. Upon the addition of AFB1, the specific aptamers are attracted to AFB1, getting distance from AuNPs which result in fluorescence recovery. Under optimized conditions the detection limit of proposed nanobiosensor was 3.4 nM with linear range of 10–400 nM. Selectivity test demonstrates that the nanobiosensor could be a promising tool for specific evaluation of food stuff. This method was successfully applied for the analysis of AFB1 in rice and peanut samples.
外文关键词：Aflatoxin B1；Aptasensor；FRET；Sensitive detection；Fluorescence；Quantum dots；Nanobiosensor；Food；Au nanoparticle
作者：Sabet, FS；Hosseini, M；Khabbaz, H；Dadmehr, M；Ganjali, MR
作者单位：Univ Tehran
期刊名称：FOOD CHEMISTRY
期刊影响因子：4.052
出版年份：2017
出版刊次：1
点击下载：基于荧光能量共振转移的适配子生物传感器用于筛选和灵敏检测花生和稻米中的黄曲霉毒素B1