中文摘要：为了研究原花青素(PAs)缓解黄曲霉毒素B1诱导的基因组损伤的潜在机制，作者审查了受PAs预处理的动物中，黄曲霉毒素B1诱导的氧化DAN损伤的调节情况。并且大鼠骨髓细胞试验检测了PAs在表达DNA损伤和修复黄曲霉毒素B1诱导的基因中的作用。研究发现，PAs保护大鼠免受黄曲霉毒素B1诱导的氧化性DAN损伤，这些保护作用归因于PAs的抗氧化作用，并且可以通过调节DNA修复基因表达来增强DNA修复。因此，PAs是一种有效的基因化学保护剂，可以避免与黄曲霉毒素B1暴露有关的基因毒性风险。
外文摘要：In order to study the mechanisms underlying the alleviation of aflatoxin B1-induced genomic damage by proanthocyanidins (PAs), we examined the modulation of oxidative DNA damage induced by aflatoxin B1 in PAs-pretreated animals. The effects of PAs on changes in the expression of DNA damage and repair genes induced by aflatoxin B1 were also evaluated in rat marrow cells. Administration of PAs before aflatoxin B1 significantly mitigated aflatoxin B1-induced oxidative DNA damage in a dose-dependent manner. Aflatoxin B1 treatment induced significant alterations in the expression of specific DNA repair genes, and the pre-treatment of rats with PAs ameliorated the altered expression of these genes. Conclusively, PAs protect against aflatoxin B1-induced oxidative DNA damage in rats. These protective effects are attributed to the antioxidant effects of PA and enhanced DNA repair through modulation of DNA repair gene expression. Therefore, PAs are a promising chemoprotective agent for averting genotoxic risks associated with aflatoxin B1 exposure.
外文关键词：GRAPE SEED EXTRACT; MURINE MARROW-CELLS; INDUCED NEPHROTOXICITY; OXIDATIVE STRESS; COMET ASSAY; EXPOSURE; LUNG; MICE; B-1; GENOTOXICITY
作者：Bakheet, Saleh A.; Alhuraishi, Ahmed M.; Al-Harbi, Naif O.; Al-Hosaini, Khaled A.; Al-Sharary, Shakir D.; Attia, Mohammed M.; Alhoshani, Ali R.; Al-Shabanah, Othman A.; Al-Harbi, Mohammed M.; Imam, Faisal; Ahmad, Sheikh F.; Attia, Sabry M.
作者单位：King Saud Univ, Egypt
期刊名称：JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
期刊影响因子：2.303
出版年份：2016
出版刊次：11
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