中文摘要：黄曲霉毒素B1（AFB1）是由一些真菌产生的毒性代谢产物，众所周知具有致肝癌和免疫抑制作用，而后者可以增加体外免疫细胞凋亡。然而究竟哪些信号通路促成了AFB1诱导的免疫细胞过度调研？这在很大程度上还是未知的。本研究中，调查了AFB1膳食暴露下肉鸡法氏囊细胞（BF）中的线粒体、内质网（ER）和激活凋亡通路的死亡受体。研究发现：（1）AFB1膳食暴露诱导BF的形态学变化。（2）FCM和TUNEL方法显示AFB1摄入可导致过度凋亡。（3）AFB1诱导的囊细胞凋亡与线粒体通路有关。（4）饲喂含AFB1的饲料后，肉鸡的法氏囊细胞被证实发生氧化应激。总体来说，本研究中证明了线粒体和ER凋亡通路的激活可以导致法氏囊细胞的过度凋亡，氧化应激是AFB1暴露期间的一个关键驱动因素。
外文摘要：Aflatoxin B1 (AFB1), a toxic metabolite produced by some fungi, exerts well-known hepatocarcinogenic and immunosuppressive effects, the latter can increase the apoptotic immune cells in vitro. However, it is largely unknown that which signaling pathways contribute to excessive apoptosis of immune cells which induced by AFB1. In this study, we investigated the roles of the mitochondria, endoplasmic reticulum (ER) and death receptor activated apoptotic pathways in the bursal of Fabricius (BF) cells in the broilers exposed to AFB1 diet. We found that (1) AFB1 diet induced morphological changes in the BF. (2) FCM and TUNEL methods showed that excessive apoptosis could be resulted from AFB1 intake. (3) AFB1-induced apoptosis of bursal cells involved mitochondrial pathway (increase of Bax, Bak, cytC, caspase-9, Apaf-1, caspase-3 and decrease of Bcl-2 and Bcl-xL) and ER pathway (increase of Grp78/Bip, Grp94 and CaM). (4) Oxidative stress was confirmed in the BF of chicken fed on AFB1 diet. Overall, this work is the first to demonstrate that the activation of mitochondria and ER apoptosis pathways can lead to excessive apoptosis in BF cells, and oxidative stress is a crucial driver during AFB1 exposure.
外文关键词：aflatoxin B-1;bursa of Fabricius;apoptosis;oxidative stress;broiler
作者：Yuan, Shibin; Wu, Bangyuan; Yu, Zhengqiang; Fang, Jing; Liang, Na; Zhou, Mingqiang; Huang, Cheng; Peng, Xi
作者单位：China West Normal Univ
期刊名称：ONCOTARGET
期刊影响因子：5.008
出版年份：2016
出版刊次：10
点击下载：与黄曲霉毒素B1膳食暴露下肉鸡的法氏囊细胞凋亡有关的线粒体和内质网路径研究