中文摘要： 本文开发了一个基于白光反射光谱法的生物传感器，该传感器可同时检测饮用水和酒水样本中毒死蜱、抑霉唑和噻苯咪唑三种农药残留。整个分析过程在10分钟内完成，毒死蜱和抑霉唑的定量限为60 pg/mL，噻菌灵的定量限为80 pg / mL。本文使用未做处理的瓶装水和经过10倍检测缓冲液稀释的白葡萄酒和红葡萄酒样本进行检测，采用这些处理方法后，毒死蜱和抑霉唑可检测浓度低至0.6 ng/mL，噻菌灵低至0.8 ng/mL。检测结果显示，批内和批间重复性变异系数分别在4.9-8.2%和7.3-9.7%范围，采用加标饮用水和葡萄酒样本的回收试验评估生物传感器测量准确性，结果显示，回收率在86%-116%范围。此外，本文分别使用该生物传感器和经过验证的LC-MS/MS方法对五种不同加标浓度水平下的葡萄酒样本进行检测，检测结果进一步证明了本文所开发生物传感器的精确性，为揭示该方法能适应食品分析领域的应用需求提供了一个良好开端。
外文摘要：The simultaneous determination of pesticides chlorpyrifos, imazalil and thiabendazole in drinking water and wine samples with a biosensor based on White Light Reflectance Spectroscopy is presented. A competitive immunoassay format was implemented followed by reaction of immunoadsorbed anti-analyte specific antibody molecules with a secondary antibody in order to enhance the signal and reduce the analysis time. The whole analysis was completed in less than 10min and the limits of quantification achieved with calibrators prepared in buffer were 60 pg/mL for chlorpyrifos and imazalil, and 80 pg/mL for thiabendazole. Bottled water samples were analysed without any treatment, whereas white and red wine samples were analysed after 10-times dilution with assay buffer. This way pesticide concentrations as low as 0.6 ng/mL for chlorpyrifos and imazalil, and 0.8 ng/mL for thiabendazole could be detected in wines. The measurements were repeatable with intra- and inter-assay CVs values ranging from 4.9 to 8.2% and from 7.3 to 9.7%, respectively, for all assays. The accuracy of the measurements was evaluated through recovery experiments using spiked water and wine samples. Percent recovery values ranged from 86 to 116%, demonstrating the accuracy of the measurements performed with the developed biosensor. In addition, 5 wines spiked with different concentrations of the three pesticides were analysed both with the developed biosensor and a validated LC-MS/MS method. The good agreement of the results obtained with the two methods is a further testimony of the developed method accuracy and a good starting point to demonstrate its Point-of-Need application capabilities in the food analysis field. (C) 2016 Elsevier B.V. All rights reserved.
 
外文关键词：White Light Reflectance Spectroscopy, Pesticides, Chlorpyrifos, Thiabendazole, Imazalil, Label-free immunosensor
作者：Koukouvinos, Georgios; Tsialla, Zoi; Petrou, Panagiota S.; Misiakos, Konstantinos; Goustouridis, Dimitrios; Moreno, Ana Ucles; Fernandez-Alba, Amadeo R.; Raptis, Ioannis; Kakabakos, Sotirios E.
作者单位：NCSR Demokritos, Inst Nuclear & Radiol Sci & Technol Energy & Safe, Immunoassay Immunosensors Lab, GR-15310 Athens, Greece.
期刊名称：SENSORS AND ACTUATORS B-CHEMICAL
期刊影响因子：4.758
出版年份：2017
出版刊次：2
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