中文摘要：本研究开发了一个经济且简单的检测乳杆菌属和金黄色葡萄球菌的免疫传感器，该传感器采用基于单克隆抗革兰氏阳性菌修饰的金纳米粒子的比色法进行检测。抗体标记的金纳米粒子较容易通过抗体识别抗原来识别革兰氏阳性细菌，并表现为纳米粒子颜色变化。纯培养下乳杆菌属和金黄色葡萄球菌的检测限分别为105 CFU/ml和120 CFU/ml。本方法成功地应用于甘蔗样本中细菌的检测且检测吻合度较高。以上结果表明，本检测系统可应用于兰氏阳性菌的定量分析，且在食品工业领域具有一定的应用潜力。
外文摘要：The presence of Gram-positive bacteria in foodstuffs is a chronic worldwide problem. Here, we present a cheap and simple colorimetric method for the detection of Lactobacillus species (spp.) and Staphylococcus aureus (S. aureus) using gold nanoparticles (AuNP) modified with monoclonal anti-Gram-positive bacteria to produce an immune-sensor. Detection is based on the fact that antibody-conjugated AuNPs can readily identify Gram-positive bacteria through antibody-antigen recognition, which results in a color change of AuNPs upon aggregation. The detection limit was 105 CFU/ml in pure culture for Lactobacillus spp. and 120 CFU/ml in pure culture for S. aureus. The method was applied successfully for detection of bacteria in samples of sugar cane, and agreed well with values obtained using other methods. These results suggested that the detection system could be used for the quantitative analysis of Gram-positive bacteria and might be applied potentially by the food industry. (C) 2016 Elsevier Ltd. All rights reserved.
 
外文关键词：Immunosensor, Bacteria, Naked eye detection
作者：Verdoodt, Niels; Basso, Caroline R.; Rossi, Bruna F.; Pedrosa, Valber A.
作者单位：UNESP Botucatu, Inst Biosci, Botucatu, SP, Brazil.
期刊名称：FOOD CHEMISTRY
期刊影响因子：4.052
出版年份：2017
出版刊次：4
点击下载：一种快速、灵敏的细菌检测免疫传感器研究