中文摘要：本研究采用混合杂交瘤技术制备了一种可同时识别甲基对硫磷和吡虫啉农药的新型双功能抗体，并将其作为唯一识别试剂，开发了一种基于时间分辨化学发光（CL）策略定量检测农药残留的复用免疫层析试纸条。甲基对硫磷和吡虫啉农药残留线性范围均为0.1-250ng/ml，检测限为0.058 ng/mL (S/N=3)。整个检测过程在22分钟内完成。试验结果显示：该方法在传统中药复方样本残留检测方面具有较大的应用潜力。本研究利用单抗技术提供了一种低成本、简便、快速的多重筛选农药残留检测方法。
外文摘要：A novel bifunctional antibody (BfAb) that could recognize methyl parathion and imidacloprid simultaneously was prepared by a hybrid hybridomas technique. Using the BfAb as the sole recognition reagent, a multiplexed immunochromatographic test strip based on a time-resolved chemiluminescence (CL) strategy was developed for quantitative detection of pesticide residues. Horseradish peroxidase (HRP) and alkaline phosphatase (ALP) were used as the CL probes to label the haptens of methyl parathion and imidacloprid, respectively. After the labeled haptens competed with methyl parathion and imidacloprid to bind with the BfAb immobilized on the test strip, the two CL reactions catalyzed by the enzymes were triggered simultaneously by coreactants injection. Due to the distinct CL kinetics characteristics of HRP and ALP, the signals for methyl parathion and imidacloprid detections were collected at 2.5 s and 300 s, respectively. The linear ranges for methyl parathion and imidacloprid were both 0.1-250 ng mL(-1), with detection limits of 0.058 ng mL(-1) (S/N=3). The whole assay process could be accomplished within 22 min. The detection results for spiked traditional Chinese medicine samples demonstrated its application potential. The proposed method provided a low-cost, facile and rapid tool for multiplexed screening of pesticide residues using single antibody.
外文关键词：Methyl parathion, Imidacloprid, Bifunctional antibody, Time-resolved chemiluminescence, Immunochromatographic test strip, Multiplexed immunoassay
作者：Shu, Qi; Wang, Limin; Ouyang, Hui; Wang, Wenwen; Liu, Fengquan; Fu, Zhifeng
作者单位：Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China.
期刊名称：BIOSENSORS & BIOELECTRONICS
期刊影响因子：7.476
出版年份：2017
出版刊次：1
点击下载：一种复用免疫层析试纸条及其与双功能抗体在基于时间分辨化学发光策略的农药检测中的应用