中文摘要:本文研究了纯橄榄酚类化合物及橄榄油厂废水(OMWW)(膜过滤处理)对黄曲霉生长和产生黄曲霉毒素B1(AFB1)的影响。结果发现所有纯化合物处于最高浓度(100mug/ml)时,对真菌的生长没有任何影响,但AFB1减少了99%。这是首次将OMWWs及其主要酚类物质用于防治AFB1。研究结果为利用橄榄多酚及OMWW抑制AFB1产生,并以可持续的方式使用橄榄油副产品,防止食品污染提供了新策略。
外文摘要:In this study, the effects of pure olive phenolic compounds and olive mill wastewater (OMWW) (after membrane filtration treatments) on Aspergillus flavus growth and aflatoxin B-1 (AFB(1)) production, were investigated. Five OMWWs coming from Greek (Lianolia, Koroneiki and Asprolia) and Italian (Cellina di Nard and Coratina) olive oil cultivars, opportunely filtered using a membrane system, were added at two concentrations (5 and 15%) to growth medium, in order to evaluate their effect on A. flavus growth and AFB(1) production. The OMWW fractions treatment, after 6 days of incubation, did not inhibit the fungal growth rate, but at 15% concentration significantly reduced the AFB(1) production (ranging from 88 to 100%). A similar approach was used for caffeic acid, hydroxytyrosol, tyrosol and verbascoside, the major pure phenolic compounds identified in OMWW fractions. They were evaluated at increasing doses (10, 50 and 100 mu g/ ml) following both AFB1 production and fungal growth. At the highest concentration (100 mu g/ ml) all pure compounds showed a reduction of about 99% of AFB(1) production without any influence on fungal growth. This is the first time in which OMWWs and their main phenolics were used in the treatments against AFB(1) production. The results obtained could provide possible new strategies for preventing AFB1 food contamination using olive polyphenols and OMWW fractions with anti-aflatoxigenic effect, and permitting to harness in a sustainable way an olive oil by-product.
外文关键词:aflatoxin B-1 inhibition;Aspergillus flavus;olive mill wastewater;polyphenols
作者:Bavaro, SL;D'Antuono, I;Cozzi, G;Haidukowski, M;Cardinali, A;Logrieco, AF
作者单位:ISPA CNR
期刊名称:World Mycotoxin Journal
期刊影响因子:2.091
出版年份:2016
出版刊次:4
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