中文摘要：本研究成功开发了一种高效液相色谱-串联质谱(HPLC-MS/MS)结合免疫亲和净化柱的方法，用于检测黄曲霉毒素( AFB1、AFB2、AFG1、AFG2、AFM1和AFM2)和玉米赤霉醇（α-玉米赤霉醇、β-玉米赤霉醇、α-玉米赤霉烯醇、β-玉米赤霉烯醇、玉米赤霉酮和玉米赤霉烯酮）。结果证明了该方法简单准确，适用于动物源性食品中黄曲霉毒素和玉米赤霉醇的快速检测。
外文摘要：A high performance liquid chromatography-tandem mass spectrometric(HPLC-MS/MS) method coupled with an immunoaffinity clean-up column was successfully developed for determination of aflatoxins( AFB_1,AFB_2,AFG_1,AFG_2,AFM_1 and AFM_2) and zeranols( α-zeranol,β-zeranol,α-zearalenol,β-zearalenol,zearalanone and zearalenone).The sample was extracted with methanol-acetonitrile( 20 ∶ 80,V/V) after enzymatic digestion by β-glucuronidase/sulfatase,and the extraction solution was passed through glassy fiber filter paper and then diluted with phosphate buffer solution( PBS).The reconstituted solution was cleaned up with IAC-AZ immunoaffinity column,and then analyzed by HPLC-MS/MS in multiple reaction monitoring( MRM) mode.The results indicated that the linear detection range was 0.03-6.0 μg/L for AFB2 and AFG2,and 0.05-20 μg /L for the rest compounds.The correlation coefficients were above 0.999.The limits of detection( LOD) and limits of quantitation( LOQ) were 0.01-0.03 μg/kg and 0.04-0.09 μg/kg,respectively.The recoveries of the aflatoxins and zeranols were in the range of 73.6%-98.4% at the spiked levels of 0.5,1 and 5 μg/kg,and the relative standard deviations( RSDs) were in the range of 1.9%-11.2%.The method was proved to be simple and accurate,and suitable for the rapid determination of aflatoxins and zeranols in animal-originated foods.
外文关键词：Animal-originated foods；Combination immunoaffinity column；Aflatoxins；Zeranols；High performance liquid chromatography-tandem mass spectrometry
作者：Xue, S；Xi, CX；Tang, BB；Wang, GM；Chen, DD；Zhao, H
作者单位：Peoples Hosp Tibet Autonomous Reg
期刊名称：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
期刊影响因子：0.554
出版年份：2016
出版刊次：6
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