中文摘要：本文对荧光偏振法进行了优化和验证，应用该方法对未加工谷物（燕麦、大麦、黑麦）和直接食用谷物制品（燕麦片、燕麦面包、面食）中T-2与HT-2毒素进行快速检测。所有添加燕麦、黑麦、大麦、燕麦、燕麦面包和面食的样品整体平均回收率在101%-107%范围，相对标准差低于7%。燕麦样品毒素残留检测限为70 μg/kg，燕麦片和大麦为40 μg/kg，面食为25 μg/kg，黑麦和燕麦面包为20μg/kg。本文以燕麦和燕麦片作为T-2与HT-2毒素参考材料对荧光偏振免疫法真实性进行评估，显示出良好的准确度和精密度。以超高效液相色谱免疫亲和柱净化法作为参考，该方法在对含有自然和人工污染的样本中T-2与HT-2毒素进行检测时表现出良好的相关性，相关系数大于0.953。结合以上结果与该方法快速、简单等优点，表明该优化分析法在高通量筛选和谷物与谷物制品中T-2、HT-2毒素的定量检测方面具有一定的适用性和可靠性。
外文摘要：A fluorescence polarization (FP) immunoassay has been optimized and validated for rapid quantification of T-2 and HT-2 toxins in both unprocessed cereals, including oats, barley and rye, and cereal-based products for direct human consumption, such as oat flakes, oats crispbread and pasta. Samples were extracted with 90 % methanol, and the extract was filtered and diluted with water or sodium chloride solution prior to the FP immunoassay. Overall mean recoveries from spiked oats, rye, barley, oat flakes, oats crispbread and pasta ranged from 101 to 107 %, with relative standard deviations lower than 7 %. Limits of detection (LODs) of the FP immunoassay were 70 mu g/kg for oats, 40 mu g/kg for oat flakes and barley, 25 mu g/kg for pasta and 20 mu g/kg for rye and oats crispbread. The trueness of the immunoassay was assessed by using two oat and oat flake reference materials for T-2 and HT-2 toxins, showing good accuracy and precision. Good correlations (r > 0.953) were observed between T-2 and HT-2 toxin contents in naturally and artificially contaminated samples determined by both FP immunoassay and ultra-high-performance liquid chromatography (UHPLC) with immunoaffinity column cleanup used as reference method. These results, combined with rapidity and simplicity of the assay, show that the optimized assay is suitable for high-throughput screening, as well as for reliable quantitative determination of T-2 and HT-2 toxins in cereals and cereal-based products.
 
外文关键词：T-2 and HT-2 toxins, Fluorescence polarization immunoassay, Oats, Barley, Rye, Cereal-based products
作者：Porricelli, Anna Chiara R.; Lippolis, Vincenzo; Valenzano, Stefania; Cortese, Marina; Suman, Michele; Zanardi, Sandro; Pascale, Michelangelo
作者单位：Natl Res Council Italy CNR, Inst Sci Food Prod ISPA, Via G Amendola 122-O, I-70126 Bari, Italy.
期刊名称：FOOD ANALYTICAL METHODS
期刊影响因子：2.167
出版年份：2016
出版刊次：12
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