中文摘要：本研究建立了一种AlphaLISA（A homogenous light-induced chemiluminescence immunoassay）方法用于测定肌肉组织样品中玉米赤霉醇及玉米赤霉烯酮的残留情况。与其它方法相比，AlphaLISA技术具有同质性、免清洗和高灵敏度等特点。实验结果表明，该方法能够成功应用于肌肉组织中玉米赤霉醇和玉米赤霉烯酮的测定。
外文摘要：A homogenous light-induced chemiluminescence immunoassay (AlphaLISA) method was established for the determination of residues of zearalanol and its analog zearalanone in muscle tissue samples. AlphaLISA is a bead-based proximity assay. When donor and acceptor beads proximity, a cascade of chemical reactions begin. The end result is a greatly amplified signal that contributes to the detection sensitivity down to the attomole level. Compared with other methods, the AlphaLISA has characteristics of homogeneity, being free of cleaning, high sensitivity. The method showed a linear relationship in the range of 0.01–4 ng/mL (R2 > 0.99); the sensitivity of the assay was 0.066 ng/mL. Cross-reactivity rate of zearalanol was 100 % and zearalanone was 82.1 %, and other compounds were not more than 40 %. The average recovery rates of Zearalanol at spiked levels of 1–4 ng/mL were 96.3 to 105.0 and 91.7 to 100.5 % for pork and bovine muscles; the intra-day precision ranged from 2.6 to 9.8 %, and the inter-day precision ranged from 8.7 to 17.5 %. These results indicated that the proposed method was successfully applied in the analysis of zearalanol and its analog zearalanone in muscle tissues.
外文关键词：AlphaLISA;Zearalanol;Zearalanone;Homogeneous
作者：Gong, Qian;Xi, Cunxian;Nie, Fuping;等
作者单位：重庆医科大学
期刊名称：FOOD ANALYTICAL METHODS
期刊影响因子：2.167
出版年份：2017
出版刊次：2
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