中文摘要：基于LC-MS/MS的多霉菌毒素检测方法的流行意味着人们对多霉菌毒素参考物质的迫切需求。本研究通过接种镰刀菌菌株获得了污染小麦面粉的多霉菌毒素，包括脱氧雪腐镰刀菌烯醇（DON）、玉米赤霉烯酮（ZEN）、T-2毒素（T-2）、HT-2毒素（HT-2）、恩镰孢菌素 A，A1，B和B1（ENNs）和白僵菌素（BEA）。该多霉菌毒素作为候选材料成功通过了同质性测试，并且提交给来自11个国家的19个实验室利用常规分析方法进行国际多实验室研究。实验表明，开发这种多霉菌毒素参考物质作为计量工具是利用多霉菌毒素分析进行食品和饲料质量控制研究的重要一步。
外文摘要：The interest in LC-MS/MS multi-mycotoxin methods unveiled an urgent need for multi-mycotoxin reference material. A multi-fusariotoxin, including deoxynivalenol (DON); zearalenone (ZEN); T-2 toxin (T-2); HT-2 toxin (HT-2); enniatin A, A1, B, and B1 (ENNs); and beauvericin (BEA), contaminated wheat flour was obtained by inoculation Fusarium spp. strains. The candidate material has successfully passed the homogeneity test and submitted to an international interlaboratory study achieved by 19 laboratories from 11 countries using their routine analytical method. The dispersion of the results for ZEN and BEA did not allow the derivation of reliable consensus values, while the assignment was only possible for DON, HT-2, T-2, and ENN A. No link was found between the methods used by the participants and the results. Significant changes in dry matter contents (≥±1.4 % of the initial dry matter) and significant changes in ergosterol contents (≥±10 %) did not occur. Using the mycotoxin contents in wheat flour stored at −80 °C as reference values, statistically significant decreases were observed only for T-2 contents at +24 °C, in contrast to the storage at −20 and +4 °C. For the other involved toxins, the candidate material was found to be stable at −20, +4, or +24 °C. Based on the T-2 decreases, a shelf life of 6 years was derived from isochronous study when the material is kept at −20 °C. At room temperature (e.g., +24 °C) or higher, this time validity drastically decreases down to 6 months. The development of this metrological tool is an important step towards food and feed quality control using multi-mycotoxin analyses. In vivo animal experiments using multi-mycotoxin-contaminated feeds dealing with the carryover or mitigation could further benefit from the methodology of this work.
外文关键词：Multi-mycotoxin；LC-MS/MS；Reference material；Cereals；Storage；Shelf life
作者：Tangni, E. K.；Debongnie, P.；Huybrechts, B.；Van Hove, F.；Callebaut, A.
作者单位：CODA CERVA, Belgium.
期刊名称：MYCOTOXIN RESEARCH
期刊影响因子：2.0
出版年份：2017
出版刊次：1
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