中文摘要：霉菌毒素脱氧雪腐镰刀菌烯醇（DON）在全世界范围内污染农产品，对人类和动物的健康造成威胁。DON的衍生物，如去环氧脱氧雪腐镰刀菌烯醇（DOM-1），由某些肠道细菌进行酶转化而产生，这些肠道细菌天然存在或可用作饲料添加剂。本研究利用分化的猪肠上皮细胞（IPEC-J2），首次对DON和DOM-1进行多参数细胞毒性比较分析，对溶酶体活性、总蛋白含量、膜完整性、线粒体代谢和ATP合成进行秉性评估。研究结果表明了利用将DON-转化成DOM-1的肠道细菌做饲料添加剂的安全性，另外，该研究还强调了广泛的多参数分析可以显著提升体外数据的质量。
外文摘要：The mycotoxin deoxynivalenol (DON) contaminates agricultural commodities worldwide, posing health threats to humans and animals. Associated with DON are derivatives, such as deepoxy-deoxynivalenol (DOM-1), produced by enzymatic transformation of certain intestinal bacteria, which are naturally occurring or applied as feed additives. Using differentiated porcine intestinal epithelial cells (IPEC-J2), we provide the first multi-parameter comparative cytotoxicity analysis of DON and DOM-1, based on the parallel evaluation of lysosomal activity, total protein content, membrane integrity, mitochondrial metabolism and ATP synthesis. The study investigated the ability of DON and—for the first time of its metabolite DOM-1—to induce apoptosis, mitogen-activated protein kinase (MAPK) signalling, oxidative events and alterations of mitochondrial structure in porcine intestinal epithelial cells (IECs). The degree of DON toxicity strongly varied, depending on the cytotoxicity parameter evaluated. DON compromised viability according to the parameters of lysosomal activity, total protein content and membrane integrity, but increased viability according to assays based on mitochondrial metabolism and ATP synthesis. DON induced expression of cleaved caspase-3 (maximum induction 3.9-fold) and MAPK p38 and p42/p44 (maximum induction 2.51- and 2.30-fold, respectively). DON altered mitochondrial morphology, but did not increase intracellular ROS. DOM-1-treated IPEC-J2 remained unaffected at equimolar concentrations in all assays, thereby confirming the safety of feed additives using DON- to DOM-1-transforming bacteria. The study additionally highlights that an extensive multi-parameter analysis significantly contributes to the quality of in vitro data.
外文关键词：Mycotoxin；IPEC-J2；Mitochondria；Apoptosis；MAPK signalling；In vitro
作者：Springler, Alexandra；Hessenberger, Sabine；Reisinger, Nicole；Kern, Corinna；等
作者单位：BIOMIN Res Ctr, Austria.
期刊名称：MYCOTOXIN RESEARCH
期刊影响因子：2.0
出版年份：2017
出版刊次：1
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