木瓜是一种提供多种维生素且具有药理作用(如免疫调节)的重要水果。抗环斑病毒转基因木瓜是通过克隆木瓜环斑病毒(PRSV)的外壳蛋白基因而形成的,其可作为抵抗PRSV感染和增加木瓜产量的有效策略。为了评估转基因木瓜作为食物的安全性,本文对Sprague Dawley(SD)大鼠喂养转基因番木瓜823-2210、其母本非转基因木瓜Tainung-29(TN-2)后的免疫反应进行了亚慢性评价研究。在喂养试验结束时,对大鼠的免疫表型、丝裂原活化脾细胞增殖、抗原抗体特异性反应、胸腺与脾脏的组织病理学进行了评价。免疫毒性检测结果显示,喂养转基因番木瓜823-2210与非转基因木瓜Tainung-2 (TN-2)大鼠在90天后没有一致的差异,这表明转基因番木瓜823-2210与非转基因木瓜Tainung-2的免疫调节反应具有实质等同性。
外文摘要:
Papaya is an important fruit that provides a variety of vitamins with nutritional value and also holds some pharmacological properties, including immunomodulation. Genetically modified (GM) papaya plants resistant to Papaya ringspot virus (PRSV) infection have been generated by cloning the coat protein gene of the PRSV which can be used as a valuable strategy to fight PRSV infection and to increase papaya production. In order to assess the safety of GM papaya as a food, this subchronic study was conducted to assess the immunomodulatory responses of the GM papaya line 823-2210, when compared with its parent plant of non-GM papaya, Tainung-2 (TN-2), in Sprague-Dawley (SD) rats. Both non-GM and GM 823-2210 papaya fruits at low (1 g/kg bw) and high (2 g/kg bw) dosages were administered via daily oral gavage to male and female rats consecutively for 90 days. Immunophenotyping, mitogen-induced splenic cell proliferation, antigen-specific antibody response, and histopathology of the spleen and thymus were evaluated at the end of the experiment. Results of immunotoxicity assays revealed no consistent difference between rats fed for 90 days with GM 823-2210 papaya fruits, as opposed to those fed non-GM TN-2 papaya fruits, suggesting that with regard to immunomodulatory responses, GM 823-2210 papaya fruits maintain substantial equivalence to fruits of their non-GM TN-2 parent.
外文关键词:immunotoxicity;subchronic;PRSV-GM papaya fruit;rats
作者:Lin, Hsin-Tang;Lee, Wei-Cheng;Tsai, Yi-Ting;等
作者单位:Natl Chung Hsing Univ
期刊名称:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
期刊影响因子:2.857
出版年份:2016
出版刊次:7
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