中文摘要：植物在响应防御相关蛋白的上调和特定代谢产物的变化时，会执行相应的机制。本文主要将表达酵母S-腺苷甲硫氨酸脱羧酶和在果实中专门积累Spd和Spm的两个转基因番茄株系（556HO和579HO）与参照番茄株系（556AZ)）作对比。高Spd和Spm的番茄与参照组的番茄被用来实验在冷害信号下多胺的作用，当果实复温到20C时，相对参照组番茄株系来说，两个转基因番茄株系果实的Spd和Spm水平进一步提高，并在复温后15天仍稳定保持高水平。在复温前和复温后对其稳态蛋白进行分析，发现类似14 kD蛋白显著。接着利用蛋白质组学、蛋白质测序和免疫印迹法，类似14 kD蛋白被确定为病程相关蛋白1B1（PR1b1）。研究发现，在参照组番茄株系中，PR1b1只有短暂的积累，且在复温到15C时几乎检测不到；在转基因番茄株系（556HO和579HO）中， PR1b1数量增加，且在复温到15C时仍保持稳定。另外，在参照组番茄株系中，PR1b1基因转录很低，且在复温到15C时才有明显的增加；在转基因番茄株系（556HO和579HO）中，PR1b1基因转录在复温后迅速积累和增加。研究结果表明，复温冷藏的番茄果实时，PR1b1的高聚胺介导持续积累是先发制人的冷应激反应，也可能是对SIDR现象的防御反应机制。
外文摘要：Plants execute an array of mechanisms in response to stress which include upregulation of defense related-proteins and changes in specific metabolites. Polyamines - putrescine (Put), spermidine (Spd), and spermine (Spm) - are metabolites commonly found associated with abiotic stresses such as chilling stress. We have generated two transgenic tomato lines (556HO and 579HO) that express yeast S-adenosylmethionine decarboxylase and specifically accumulate Spd and Spm in fruits in comparison to fruits from control (556AZ) plants (Mehta et al., 2002). Tomato fruits undergo chilling injury at temperatures below 13 degrees C. The high Spd and Spm tomato together with the control azygous line were utilized to address role(s) of polyamines in chilling-injury signaling. Exposure to chilling temperature (2 degrees C) led to several-fold increase in the Put content in all the lines. Upon re-warming of the fruits at 20 degrees C, the levels of Spd and Spm increased further in the fruit of the two transgenic lines, the higher levels remaining stable for 15 days after re-warming as compared to the fruit from the control line. Profiling their steady state proteins before and after re-warming highlighted a protein of similar to 14 kD. Using proteomics approach, protein sequencing and immunoblotting, the similar to 14 kD protein was identified as the pathogenesis related protein 1b1 (PR1b1). The PR1b1 protein accumulated transiently in the control fruit whose level was barely detectable at d 15 post-warming while in the fruit from both the 556HO and 579HO transgenic lines PR1b1 abundance increased and remained stable till d 15 post warming.  PR1b1 gene transcripts were found low in the control fruit with a visible accumulation only on d 15 post warming; however, in both the transgenic lines it accumulated and increased soon after rewarming being several-fold higher on day 2 while in 556HO line this increase continued until d 6 than the control fruit. The chilling-induced increase in PR1b1 protein seems independent of ethylene and methyl jasmonate signaling but may be linked to salicylic acid. We propose that polyamine-mediated sustained accumulation of PR1b1 protein in post-warmed chilled tomato fruit is a pre-emptive cold stress response and possibly a defense response mechanism related to Cold Stress-Induced Disease Resistance (SIDR) phenomenon.
外文关键词：low temperature response；Solanum lycopersicum；cold responsive genes；methyl jasmonate；PR proteins；genetic engineering；1-MCP； ethylene
作者：Goyal, Ravinder K；Fatima, Tahira；Topuz, Muhamet；等
作者单位：美国农业部
期刊名称：FRONTIERS IN PLANT SCIENCE
期刊影响因子：4.495
出版年份：2016
出版刊次：6
点击下载：病程相关蛋白1b1（PR1b1）是在高聚胺转基因番茄果实中蛋白质对低温和上调的主要响应