中文摘要：本文用光电化学（PEC）传感器，开发了一种检测花椰菜花叶病毒35S启动子（CaMV35S）的方法来区分转基因与非转基因大豆样品。在该PEC生物传感系统中，分别将制备的纳米金-还原氧化石墨烯作为固定巯基功能探针的纳米载体、二氧化硅@ CdTe量子点（QDs）核壳纳米粒子标记的氨基功能探针作为信号指标。研究结果表明，本文研发的PEC生物传感器可成功地鉴别转基因大豆与非转基因大豆，且与聚合酶链反应（PCR）的检测结果一致，这表明该PEC生物传感器是未来日常转基因检测的一种可行工具。
外文摘要：A methodology for detection of the Cauliflower Mosaic Virus 35S(CaMV35S) promoter was developed to distinguish transgenic from non-transgenic soybean samples by using photoelectrochemical (PEC) biosensor. In this PEC biosensing system, the as-prepared gold nanoparticles-reduced graphene oxide acted as a nanocarrier to immobilize the thiol-functional probe (probel), and the SiO2@CdTe quantum dots (QDs) core-shell nanoparticles tagged with the amino-functional probe (probe2) acted as signal indicators, respectively. In the presence of target DNA (tDNA) of CaMV35S, the binding of tDNA with probel and probe2 through the high specific DNA hybridization led to the fabrication of sandwich structure, and thus the high loading of the signal indicators SiO2@CdTe QDs at the electrode surface, which increased the PEC signal. The increased PEC signal depended on the concentration of tDNA, and a wide linear range from 0.1 pM to 0.5 nM with low detection limit of 0.05 pM was obtained. In addition, the PEC biosensor has been successfully used for discriminating transgenic soybean from non-transgenic samples, which was consistent with the polymerase chain reaction (PCR) results, suggesting the proposed PEC biosensor is a feasible tool for the further daily genetically modified organism detection.
外文关键词：Transgenic soybean；Photoelectrochemical biosensor；Silica coated CdTe quantum dot； Gold nanoparticles-reduced graphene oxide
作者：Li, Yaqi；Sun, Li；Liu, Qian；等
作者单位：江苏大学
期刊名称：TALANTA
期刊影响因子：4.035
出版年份：2016
出版刊次：12
点击下载：以二氧化硅@ CdTe量子点的纳米粒子为信号指标，光电化学生物传感器可鉴别转基因大豆与非转基因大豆