中文摘要：越来越多的研究凸显了气候变化对产毒曲霉产黄曲霉毒素的发生和流行的影响。人们担心急性和慢性暴露后，对人体健康和动物生产能力产生毒性效应，影响将来为全球提供安全、充足的食物的能力。已有大量的研究致力于根据这些毒素的理化和生化特性，检测人、畜消费的农产品中的黄曲霉毒素复合物。随着食品安全的改进，全球涌现了许多黄曲霉毒素可接受范围的限定，其中以欧洲最严。这些限定对发展中国家非常重要，因为黄曲霉毒素的发生严重影响着国际贸易和经济。发达国家拥有成熟的检测方法，能得到精准度很高的检测结果，适用于实验室监管。然而，许多国家遭受着黄曲霉毒素污染却没有HPLC和MS等高新技术设施，需要更加经济、稳健而精确的替代物供新兴经济体的生产者、加工者和贸易者使用。对于那些想要在发达国家开拓利润高、管理严的新市场的企业，拥有确保出口商品能满足消费者质量安全需要的检测方法尤其重要。本研究评估了用ToxiMet系统替代UPLC–MS/MS方法，根据现行的欧洲监管标准检测和测定黄曲霉毒素。结果发现，ToxiMet系统可以用于现行规定的黄曲霉毒素的检测，但鉴于本研究特别是在玉米和花生中检测到高水平的黄曲霉毒素M1的情况，因此需要考虑这些商品中某些毒素的监管水平。
 
外文摘要：Increasing research has highlighted the effects of changing climates on the occurrence and prevalence of toxigenic Aspergillus species producing aflatoxins. There is concern of the toxicological effects to human health and animal productivity following acute and chronic exposure that may affect the future ability to provide safe and sufficient food globally. Considerable research has focused on the detection of these toxins, based on the physicochemical and biochemical properties of the aflatoxin compounds, in agricultural products for human and animal consumption. As improvements in food security continue more regulations for acceptable levels of aflatoxins have arisen globally; the most stringent in Europe. These regulations are important for developing countries as aflatoxin occurrence is high significantly effecting international trade and
the economy. In developed countries analytical approaches have become highly sophisticated, capable of attaining results with high precision and accuracy, suitable for regulatory laboratories. Regrettably, many countries that are affected by aflatoxin contamination do not have resources for high tech HPLC and MS instrumentation and require more affordable, yet robust equally accurate alternatives that may be used by producers, processors and traders in emerging economies. It is especially important that those companies wishing to exploit the opportunities offered by lucrative but highly regulated markets in the developed world, have access to analytical methods that will ensure that their exports meet their customers quality and safety requirements.This work evaluates the ToxiMet system as an alternative approach to UPLC–MS/MS for the detection and determination of aflatoxins relative to current European regulatory standards. Four commodities: rice grain, maize cracked and flour, peanut paste and dried distillers grains were analysed for natural aflatoxin contamination. For B1 and total aflatoxins determination the qualitative correlation, above or below the regulatory limit, was good for all commodities with the exception of the dried distillers grain samples for B1 for which no calibration existed. For B1 the quantitative R-2 correlations were 0.92, 0.92, 0.88 (<250 μg/kg) and 0.7 for rice, maize, peanuts and dried distillers grain samples respectively whereas for total aflatoxins the quantitative correlation was 0.92, 0.94, 0.88 and 0.91. The ToxiMet system could be used as an alternative for aflatoxin analysis for current legislation but some consideration should be given to aflatoxin M1 regulatory levels for these commodities considering the high levels detected in this study especially for maize and peanuts.
 
外文关键词：ToxiMet；UPLC-MS/MS；Aflatoxin；Food and feed；Spectroscopy；Aflatoxin M1
作者：Campbell, K；Cavalcante, ALF；Galvin-King, P；Oplatowska-Stachowiak, M；Brabet, C; Metayer, I；Montet, D；Haughey, SA；Elliott, CT
作者单位：Queens Univ Belfast
期刊名称：SENSORS AND ACTUATORS B-CHEMICAL
期刊影响因子：4.758
出版年份：2017
出版刊次：2
点击下载：一种可替代光谱法的黄曲霉毒素分析方法：以食品和饲料为样品，与UPLC-MS/MS法进行比较分析