中文摘要：黄曲霉毒素是潜在的有毒代谢物，由食品中的真菌产生。因此，检测霉菌毒素如黄曲霉毒素的含量具有重大的药用价值。本研究报道了用酶联免疫吸附法（ELISA），一步一步可视化检测花生和玉米食品样品中的黄曲霉毒素。结果表明，这种可视化演示显示了ELISA法检测各种食品中黄曲霉毒素含量的重要性和适用性，避免了各种分析问题，如取样、繁琐的样品制备和关键分析方法的选择等。
外文摘要：Aflatoxin are potentially toxic metabolites produced by microscopic fungi when grow on food. Hence, determining content of mycotoxins such as aflatoxin has significant pharmaceutical value. This study reports step-by-step visual demonstration of determination of aflatoxin contents in peanut and maize (corn)-based food samples through employing enzyme linked immunosorbent assay (ELISA). The method is based on direct competitive ELISA in a micro-well format which allows determination of exact amount of aflatoxin in food samples as parts per billion (ppb), and the results are calculated using Neogen’s Veratox data reduction software that converts the absorbance values into quantities. This visual experiment will certainly contribute knowledge regarding importance and applicability of the ELISA method for determining aflatoxin content in variety of food samples avoided of analytical issues such as sampling, laborious sample preparation, and critical selection of analytical methods.
作者：Shukla, S
作者单位：Yeungnam Univ
期刊名称：BANGLADESH JOURNAL OF PHARMACOLOGY
期刊影响因子：0.671
出版年份：2016
出版刊次：3
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