中文摘要：目前，因多种霉菌毒素在食品原料中同现使毒性增强，迫切需要多霉菌检测的方法。本研究开发了磁控传感器，用于同时电化学检测赭曲霉素A（OTA）和伏马菌素B1（FB1）这两种全球粮食中典型的霉菌毒素。本方法提供了一种新的高通量筛选霉菌毒素的方法，具有装置简单、样品消耗低、耗时短及成本低的特点。此外，它还容易扩展，只要提供特殊的适配子，用不同的硫化金属量子点标记，就可用于多种霉菌毒素的同时检测。
外文摘要：

Currently there is an urgent need for multi-mycotoxin detection methods due to the co-occurrence of multiple mycotoxins in food raw materials and their augmented toxicity. Herein, a magneto-controlled aptasensor has been developed for simultaneous electrochemical detection of ochratoxin A (OTA) and fumonisin B1 (FB1), two typical mycotoxins found in food crops world-wide. This aptasensor was designed using the high specificity between the target and aptamer with heavy CdTe or PbS quantum dots (QDs) coated silica as labels and the complementary DNA functionalized magnetic beads as capture probes. In presence of targets, the aptamer preferred to form the target-aptamer binding which forced the partial release of the preloaded labels from the magnetic beads. After a one-step incubation and a simple magnetic separation, the electrochemical signals of Cd2+ and Pb2+ dissolved from the reserved labels which had negative correlation with targets contents, was measured based on the difference of peak potentials. This aptasensor provided a wide detection range of 10 pg mL(-1) to 10 ng mL(-1) for OTA and 50 pg to 50 ng mL(-1) for FBI, and succeeded in real maize samples. This method provides a new avenue for high throughput screen of mycotoxins due to the advantages of simple instrument, low sampleconsumption, short assay times, and lower detection costs per assay. Moreover, it could be readily expanded for the simultaneous detection of a large panel of mycotoxins by using different metal sulfide QDs when their specific aptamers are available.Keywords:Electrochemical aptasensor, Magneto-controlled, Simultaneous detection, Mycotoxins, Ochratoxin A, Fumonisin B1

作者：Wang, CQ；Qian, J；An, KQ；Huang, XY；Zhao, LF；Liu, Q；Hao, N；Wang, K
作者单位：Jiangsu Univ
期刊名称：BIOSENSORS & BIOELECTRONICS
期刊影响因子：7.476
出版年份：2017
出版刊次：3
点击下载：以硫化金属量子点包被二氧化硅为标记的磁控传感器用于同时电化学检测玉米中的两种霉菌毒素