中文摘要：本文通过对食蟹猴的52周饲喂研究，来评价表达CrylAb/lAc蛋白的转基因水稻的安全性。作者分别对猴子饲喂含20%或60% Huahui 1的饮食、含20%或60% Minghui 63的饮食、正常饮食、正常饮食中加入纯化的重组Cry1Ab/1Ac融合蛋白或牛血清白蛋白。在饲喂试验期间，作者每天进行临床观察，并对包括体重、体温、心电图、血液学、血液生化、微生物、血清代谢、和肠道微生物等多个参数进行定期检查。在食蟹猴死后，对其器官进行宏观、微观和电子显微镜检查。研究结果表明，表达CrylAb/lAc蛋白的转基因水稻对猴子没有不良或有毒的的影响。因此，52周的灵长类动物饲喂研究表明，表达CrylAb/lAc蛋白的转基因水稻与其亲本水稻Minghui 63的安全性相当。
外文摘要：A 52-week feeding study in cynomolgus macaques was carried out to evaluate the safety of Bt rice Huahui 1 (HH1), a transgenic rice line expressing CrylAb/lAc protein. Monkeys were fed a diet with 20% or 60% HH1 rice, 20% or 60% parental rice (Minghui 63, MH63), normal diet, normal diet spiked with purified recombinant Cry1Ab/1Ac fusion protein or bovine serum albumin (BSA) respectively. During the feeding trail, clinical observations were conducted daily, and multiple parameters, including body weight, body temperature, electrocardiogram, hematology, blood biochemistry, serum metabolome and gut microbiome were examined at regular intervals. Upon sacrifice, the organs were weighted, and the macroscopic, microscopic and electron microscopic examinations were performed. The results show no adverse or toxic effects of Bt rice HUH or Cry1Ab/lAc fusion protein on monkeys. Therefore, the present 52 -week primate feeding study suggests that the transgenic rice containing Cry 1Ab/lAc is equivalent to its parental rice line MH63.
外文关键词：Genetically modified rice; Cynomolgus macaque; Bacillus thuringiensis; Safety assessment
作者：Mao, Jie; Sun, Xing; Cheng, Jian-Hua; 等
作者单位：Natl Ctr Biomed Anal
期刊名称：FOOD AND CHEMICAL TOXICOLOGY
期刊影响因子：3.584
出版年份：2016
出版刊次：9
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