中文摘要：含有199个转基因事件的转基因作物特异性矩阵，被开发用来筛选全球批准的转基因玉米和棉花事件，其包括有75个遗传元素的143个转基因玉米事件和有45个遗传元素的56个棉花事件。多重PCR技术的实用性已被六个玉米样品的和八个棉花样品的能力测试证实。四、五重PCR技术可以有效地筛选出93%全球批准的玉米事件和94%全球批准的棉花事件。转基因作物矩阵与多重PCR相结合可便于检查种子样品或食品、饲料产品中转基因作物的存在，并检测食品和供应链中批准的转基因作物的存在。这种方法可以很容易地在发展中国家的转基因作物测试实验室中使用，这是因为多重PCR技术不仅易于操作、且时间和成本投入少。本文提出的转基因作物矩阵是基于转基因玉米事件和转基因棉花事件的当前信息，它可包括新批准的转基因事件来进一步升级。由于大多数新批准的转基因事件是已商业化转基因事件的堆叠品种，因此本文开发的多重PCR技术也可以用来筛选他们的存在。
外文摘要：Crop-specific GMO matrices of 199 genetically modified (GM) events, comprising 143 GM maize events with 75 genetic elements and 56 cotton events with 45 genetic elements, were developed to screen globally approved GM maize and cotton events. As per the compiled information in the matrix, frequently present genetic elements were identified using GMOSeek algorithm: eight genetic elements ([P-35S] [r-act] [T-35S] [T-nos] [pinII] [pat] [aad-1] [cp4 epsps]) in maize and four ([P-35S] [T-nos] [pat] [nptII]) in cotton. Based on the cost-efficiency, feasibility of plexing and coverage of GM events, maize specific tetraplex PCR, targeting Cauliflower Mosaic Virus 35S promoter (P-35S), Agrobacterium tumefaciens nos terminator (T-nos), Cauliflower Mosaic Virus 35S terminator (T-35S) and endogenous alcohol dehydrogenase (Adh1) gene, with limit of detection (LOD) up to 0.5% was developed. For screening of GM cotton events, pentaplex PCR, targeting P-355, T-nos, neomycin phosphotransferase II (nptII), phosphinothricin acetyltransferase (pat) and endogenous stearoyl-ACP desaturase (Sad]) gene, with LOD up to 0.25% was developed. Practical applicability of multiplex PCR assays was confirmed with six maize samples of proficiency testing and eight spiked cotton samples. The reported tetraplex and pentaplex PCR assays could efficiently screen 94% of maize and 93% of cotton events approved globally. The developed GMO matrices in combination with multiplex PCR could facilitate checking the GM status of seed samples or food and feed products, and monitoring for presence of authorized GMO5 in food and supply chain. This approach can be easily employed by low resource GM testing laboratories in the developing countries, as the multiplex PCR assays are easy to operate with less time and cost inputs. The GMO matrices being presented herein are based on the current information of approved GM events of maize and cotton, which can be further upgraded by including new approved GM events. As most of the newly approved GM events are stacked versions of already commercialized GM events, the developed multiplex PCR assays could also be employed to screen for their presence.
外文关键词：Genetically modified (GM) events; Cotton; Crop-specific GMO matrix; Maize; Multiplex PCR; Screening assays
作者：Singh, Monika; Bhoge, Rajesh K.; Randhawa, Gurinderjit
作者单位：Natl Bur Plant Genet Resources
期刊名称：FOOD CONTROL
期刊影响因子：3.388
出版年份：2016
出版刊次：12
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