中文摘要：作者提出涂有金纳米粒子的SPR传感器芯片可高灵敏地检测转基因作物。检测是基于局域表面等离子体共振（LSPR）及其已知的敏感性和折射率的微小变化。该装置包含卤素光源、光探测器，和含有涂金纳米粒子传感器芯片的比色皿细胞。它在光路的传输模式中操作来增强等离子体信号。研究发现，该传感器芯片具有反应时间短、易于操作、可移植性、可现场检测和现场测试的性能。
外文摘要：The authors describe an SPR sensor chip coated with gold nanoparticles (AuNPs) that enables highly sensitive determination of genetically modified (GM) crops. Detection is based on localized surface plasmon resonance (LSPR) with its known sensitivity to even minute changes in refractive index. The device consists of a halogen light source, a light detector, and a cuvette cell that contains a sensor chip coated with AuNPs. It is operated in the transmission mode of the optical path to enhance the plasmonic signal. The sample solution containing target DNA (e.g. from the GM crop) is introduced into the cuvette with the sensor chip whose surface was functionalized with a capture DNA. Following a 30-min hybridization, the changes of the signal are recorded at 540 nm. The chip responds to target DNA in the 1 to 100 nM concentration range and has a 1 nM detection limit. Features of this sensor chip include a short reaction time, ease of handling, and portability, and this enables on-site detection and in-situ testing.
外文关键词：Genetically modified organism; Biosensor; Sensor chip; Nanoplasmonic device; LSPR; CP4 EPSPS gene; Herbicide resistance; DNA hybridization; Spectrophotometer
作者：Jang, Huisoo; Kwak, Cheol Hwan; Kim, Gibum; 等
作者单位：Inha Univ
期刊名称：MICROCHIMICA ACTA
期刊影响因子：4.831
出版年份：2016
出版刊次：9
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