中文摘要：黄曲霉毒素M1(AFM1)，牛奶中常见，是一组全球共同关注的2b类致癌物。人们用各种微生物结合AFM1，减少其在人体中的生物利用率。本文研究了三株乳酸菌、一株酿酒酵母和以上四种菌的复合物结合AFM1的能力。结果表明，用微生物能有效地将牛奶及乳制品中的AFM1降到安全范围。
外文摘要：Aflatoxin M1 (AFM1) is a group 2b category carcinogenic compound of global concern due to its occurrence in milk. Various microbes have been employed for the binding of AFM1 and to reduce its bioavailability in humans. In the current study three strains of lactic acid bacteria, a strain of Saccharomyces cerevisiae and a mixture of all four were used to evaluate their binding potentials for AFM1. Milk samples were spiked with two different concentrations of AFM1, 0.05 and 0.1 μg/l, and four concentrations of microbes, 107, 108, 109 and 1010 cfu/ml, were tested to evaluate their binding potentials. The concentration of AFM1 and microbes were found to significantly affect the binding potentials of microbes. Saccharomyces cerevisiae, Lactobacillus helveticus and the mixture of microbes at the concentration of 1010 cfu/ml resulted in 100% binding of AFM1. Lactobacillus helveticus was found to have a higher binding potential than any other lactic acid bacteria reported previously. The binding of AFM1 with microbes was reversible as the washing test resulted in ∼20–∼70% release of AFM1. These results indicate that the microbes can be effectively used for the reduction of AFM1 levels up to safe limits in milk and milk products.
外文关键词：Aflatoxin M-1；Milk；ELISA；Binding；Washing
作者：Ismail, A；Levin, RE；Riaz, M；Akhtar, S；Gong, YY；de Oliveira, CAF
作者单位：Bahauddin Zakariya Univ
期刊名称：FOOD CONTROL
期刊影响因子：3.388
出版年份：2017
出版刊次：3
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