中文摘要：黄曲霉毒素M1（AFM1），一种黄曲霉毒素B1的肝代谢产物，是潜在的致癌物，常见于牛奶、乳酪及其它乳制品中。本研究旨在阐明手工制作和储存期间，新鲜乳酪和乳清中AFM1的分布和去向，以及这些毒素与各种牛奶、乳酪和乳清蛋白间相互作用的水平。此外，还分析了共价交联后，酪蛋白、α-乳清蛋白、β-乳球蛋白、牛血清白蛋白(BSA)、乳铁蛋白及以上混合物在体外与固定浓度的AFM1的反应。本研究结果为乳制品行业提高乳酪及其它乳制品的安全性提供了干预依据。
外文摘要：Aflatoxin M1 (AFM1), a metabolite resulting from the hepatic metabolism of aflatoxin B1, is a potential carcinogen that can be found in milk, cheese, and others dairy products. In this work, we aimed to elucidate the distribution and fate of AFM1 in fresh cheese and whey during cheese manufacturing and storage as well as the level of interaction of this toxin with various milk, cheese, and whey proteins. Additionally, we analyzed the in vitro behavior of casein, α-lactalbumin, β-lactoglobulin, bovine serum albumin (BSA), lactoferrin, and mixtures thereof, with a fixed concentration of AFM1 after covalent crosslinking. Our results show that up to 70% of the AFM1 levels present in milk spiked with 0.5 and 1.5 μg L−1 are released in whey during fresh cheese manufacturing. The whey and milk proteins with more AFM1 molecules bound were α-lactalbumin and casein, with 88% and 81%, respectively. We also observed a substantial decrement in AFM1 concentration during ripening that correlated inversely with plate counts of lactic acid bacteria and directly with the whey-draining process that occurs during fresh cheese maturation or storage. This knowledge may serve as a basis for interventions in the dairy industry aiming to increase the security of cheese and other dairy products.
外文关键词：Aflatoxin M-1；Fresh cheese；Milk；Whey；Cheese manufacturing；Milk protein interactions
作者：Chavarria, G； Molina, A；Leiva, A； Mendez, G； Wong-Gonzalez, E； Cortes-Munoz, M； Rodriguez, C； Granados-Chinchilla, F
作者单位：Escuela Tecnol Alimentos
期刊名称：FOOD CONTROL
期刊影响因子：3.388
出版年份：2017
出版刊次：3
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