中文摘要：有毒真菌代谢物黄曲霉毒素B1（AFB1）是一种稳定的致癌物，在食品如花生和辣椒中时有发现。本研究中，将AFB1涂布到盖玻片上，用源于1.5kpps（千脉冲每秒）的静态感应晶闸管的短高压脉冲产生的氮气等离子体处理。结果显示热量和紫外光不足以灭活毒素，但处理过程中产生的化学物质有助于降解AFB1。因此控制和优化产生等离子体的条件，促进这些化学物质生成，能更有效地灭活AFB1。
外文摘要：Toxic fungal metabolite aflatoxin B1 (AFB1) is a stable carcinogen that is sometimes found in foods, such as peanuts and peppers. In this study, AFB1 was applied to a coverglass and subjected to treatment with nitrogen gas plasma generated by a plasma apparatus using a short high voltage pulse from a static induction thyristor power supply at 1.5 kpps (kilo pulse per second). Enzyme-linked immunosorbent assay showed that a 20 μL aliquot of a 200 ppb solution of AFB1 was efficiently degraded to less than one tenth of the original level within 15 min. High-performance liquid chromatography confirmed the loss of AFB1 after plasma treatment and the generation of small fragments, possibly originating from the degradation process. Moreover, a cell-based assay using HepG2 as an index to measure the cell-growth promoting properties of AFB1 showed that the gas plasma treatment reduced the biological activity of the mycotoxin. Although the amount of heat and ultraviolet light is insufficient to inactivate the toxin, the reactive chemical products appear to contribute to the degradation of AFB1. Controlling and optimizing the conditions for producing these reactive chemical species during plasma generation would lead to efficient inactivation of AFB1.
外文关键词：Non-thermal gas plasma； Discharge； Static induction thyristor； Mycotoxin；Oxidative stress
作者：Sakudo, A；Toyokawa, Y；Misawa, T； Imanishi, Y
作者单位：Univ Ryukyus
期刊名称：FOOD CONTROL
期刊影响因子：3.388
出版年份：2017
出版刊次：3
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