中文摘要：本文用环介导等温扩增（LAMP）方法开发了一种直观且快速检测MON88017玉米的分析方法。MON88017玉米的LAMP方法仅需40分钟就可完成检测，且LAMP检测灵敏度与常规PCR法在相同水平。LAMP扩增物可以在加入SYBR Green I后用肉眼检查直接检测。研究结果表明，LAMP方法具有直观、快速、灵敏度高的特点，且与传统的PCR技术相比不需要特殊的设备，这使它成为转基因作物现场试验和快速筛选的一个高效方法。
外文摘要：In this study, we developed a visual and rapid assay for the detection of MON88017 maize using the LAMP method. The LAMP method was specific for MON88017 event and takes only 40 min and the LAMP assay sensitivity is about 40 copies, which is the same level as that of conventional PCR method. LAMP amplicons can directly be detected by naked-eye inspection after adding SYBR Green I. In summary, the LAMP method is visual, faster, and more sensitive and does not need special equipment compared to the traditional PCR technique, which makes it a very higher efficiency approach for field tests and fast screening of GMO crops, especially for on-site, large-scale testing purposes in the field.
外文关键词：GMO; LAMP; MON88017; Detection
作者：Zhen, Zhen; Zhang, Minghui; Yu, Yanbo; 等
作者单位：Northeast Agr Univ
期刊名称：EUROPEAN FOOD RESEARCH AND TECHNOLOGY
期刊影响因子：1.433
出版年份：2016
出版刊次：10
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