中文摘要：本发明公开了一种辅助检测食品中是否存在转基因蛋白质的方法，包括：提取总蛋白；将总蛋白质注射到检测装置的加样孔，封闭第一多孔板体上的孔洞，该检测装置包括：第一多孔板体；加样装置，其包括第二多孔板体、圆锥体和多条加样通道；中空纤维聚丙烯膜管，其设置于第一多孔板体和第二多孔板体之间，内壁上附着有与待检测转基因蛋白质特异性结合的第一抗体；打开第一多孔板体上的孔洞，洗涤；再次封闭第一多孔板体上的孔洞，将第二抗体注射到检测装置的加样孔；再次打开第一多孔板体上的孔洞，洗涤；检测中空纤维聚丙烯膜上是否存在第二抗体的标记，若存在标记，则认为该总蛋白中存在该转基因蛋白，并判断该原材料中存在转基因成分。
外文摘要：NOVELTY - Assisting detection of presence or absence of transgenic protein in food product involves extracting the total protein of raw materials. The total protein is injected into sampling port of detecting unit so that total protein passes through detecting unit. The detecting unit comprises first perforated plate body that is provided horizontally, sampling unit having a second perforated plate body, a cone that is disposed on second perforated plate body and forms an accommodating space.
USE - Method for assisting detection of presence or absence of transgenic protein in food product (claimed).
DETAILED DESCRIPTION - Assisting detection of presence or absence of transgenic protein in food product involves extracting the total protein of raw materials. The total protein is injected into sampling port of detecting unit so that total protein passes through detecting unit. The detecting unit comprises first perforated plate body that is provided horizontally, sampling unit having a second perforated plate body, a cone that is disposed on second perforated plate body and forms an accommodating space. The second perforated plate is disposed parallel to first perforated plate and is located below first perforated plate, and its diameter is gradually reduced from bottom to top, and its free end forms a sampling hole. The multiple sampling channels are arranged one by one with sampling holes in second perforated plate body, and one end of multiple sampling channels are communicated with sampling hole. The other end of multiple sampling channels are communicated with holes in second perforated plate body one by one. The hollow fiber polypropylene film tube is disposed between first perforated plate body. The hollow fiber polypropylene tube is attached to inner wall of hollow fiber polypropylene tube, and upper end of hollow fiber polypropylene film tube is fixed to inner wall of hollow fiber polypropylene film tube. The lower end of perforated plate is fixed in holes in first perforated plate, and multiple protrusion unit is provided on inner wall of hollow fiber polypropylene film tube so that it closes holes in first perforated plate body, and when total protein passes through detecting unit then total protein is carried out between upper end of multiple hollow fiber polypropylene films. The membrane pores of hollow fiber polypropylene film and transgenic protein are bounded to first antibody. The pores in first perforated plate are opened to wash residue in the cavity of hollow fiber polypropylene film. The second antibody labeled with transgenic protein is injected into sampling port of detection unit so that second antibody passes through it. The pores of first perforated plate body are opened, residue in cavity of the hollow fiber polypropylene film are washed. The presence or absence of label of second antibody on hollow fiber polypropylene film are detected, and if label is present then it is considered that transgenic protein is present in total protein and it is determined that transgenic protein is present in the raw material.
TF   TECHNOLOGY FOCUS - BIOLOGY - Preferred Conditions: The pores of first perforated plate body are closed and opened by utilizing sealing film. The label is horseradish peroxidase. The detection is performed by adding p-dimethylaminoazobenzene to hollow fiber polypropylene film to produce a brown precipitate, and then it is judged that the label is present on hollow fiber polypropylene film. The obtained total protein is sucked into a syringe, and syringe is inserted into sample wells to inject total protein into the detection unit. The raw material comprises corn, rice, soybean and wheat. The hollow fiber polypropylene film tube and first perforated plate body have a beta angle of 70 degrees C and 90 degrees C. The hollow fiber polypropylene film tube is formed in a curvilinear shape on first and second perforated plates. The first and second perforated plates have rectangular shape, and their holes are arranged in a straight line. The first and second perforated plates are rounded, and their holes are arranged in circumferential direction.
主权项：一种辅助检测食品中是否存在转基因蛋白质的方法，其特征在于，包括：步骤一、提取原材料的总蛋白；步骤二、将步骤一得到的总蛋白质注射到检测装置的加样孔，使总蛋白质通过所述检测装置，该检测装置包括：第一多孔板体，其水平设置；加样装置，其包括第二多孔板体、设置于所述第二多孔板体上并与其形成一容置空间的圆锥体和多条加样通道，所述第二多孔板体与所述第一多孔板体平行设置，且位于所述第一多孔板体的下方，所述圆锥体的直径由下到上逐渐减小且其自由端形成加样孔，所述多条加样通道与所述第二多孔板体上的孔洞一一对应设置，且所述多条加样通道的一端均与所述加样孔连通，而所述多条加样通道的另一端均与所述第二多孔板体上的孔洞一一对应连通；中空纤维聚丙烯膜管，其设置于所述第一多孔板体和所述第二多孔板体之间，所述
申请号：CN201610756606.7
专利号：CN106324258-A
公开/公告号：CN106324258-A
申请/专利权人：黎志春
发明/设计人：黎志春
分类号：G01N33/68;G01N33/545
主分类号：G01N33/68
法律状态：实质审查
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