中文摘要：本发明涉及转基因作物安全评价领域，一种评价物理隔离措施对转基因棉花基因漂移频率影响的方法，包括如下步骤：沿温室长边方向顺序设置转基因棉花区，隔离区和常规棉区；隔离区和常规棉区沿温室短边方向划分为N条，将其中一条的隔离区作为空白带，其余条的隔离区作为隔离带，隔离带以待测的物理隔离措施进行隔离；开花期从转基因棉花区面向常规棉区送风，开花期结束后，沿转基因棉花区至常规棉区的线性方向检测是否存在外源基因，评价物理隔离措施对转基因棉花基因漂移频率影响。本发明能比较几种物理隔离措施对转基因作物基因漂移效果的影响。
外文摘要：NOVELTY - Evaluating the effect of physical isolation on the frequency of genetic drift of a transgenic cotton involves setting transgenic cotton area, isolation area and the conventional cotton area in the greenhouse; dividing isolation area and conventional cotton area into N strips, where one strip of the isolation area is taken as the blank band and rest of the isolation area as isolation band where transgenic cotton area is facing the conventional cotton area for flowering, maintaining the conventional cotton and transgenic cotton planting density; then maintaining light, and temperature.
USE - Method for evaluating effect of physical isolation on the frequency of genetic drift of transgenic cotton (claimed); and for comparing the effects of multiple physical isolation method on the genetic drift of the transgenic crop.
DETAILED DESCRIPTION - Evaluating the effect of physical isolation on the frequency of genetic drift of a transgenic cotton involves setting transgenic cotton area, isolation area and the conventional cotton area in the greenhouse; dividing isolation area and conventional cotton area into N strips, where one strip of the isolation area is taken as the blank band and rest of the isolation area as isolation band where transgenic cotton area is facing the conventional cotton area for flowering, maintaining the conventional cotton and transgenic cotton planting density; maintaining light, temperature, humidity and other environmental factors during plant growth stage; taking filial 1 hybrid (F1) sample from conventional cotton plant, after flowering, to detect the presence of foreign genes; determining the effect of different isolation methods on the frequency of genetic drift. An INDEPENDENT CLAIM is included for greenhouse for evaluating the effect of physical isolation on the frequency of genetic drift of a transgenic cotton, which comprises transgenic cotton area, isolation area and the conventional cotton area; and air supplying device for providing air to transgenic cotton area and conventional cotton air.
主权项：一种评价物理隔离措施对转基因棉花基因漂移频率影响的方法，其特征在于，包括如下步骤：1)沿温室长边方向顺序设置转基因棉花区，隔离区和常规棉区；隔离区和常规棉区沿温室短边方向划分为N条，将其中一条的隔离区作为空白带，其余条的隔离区作为隔离带，隔离带以待测的物理隔离措施进行隔离；2)开花期从转基因棉花区面向常规棉区送风，此外保持常规棉区和转基因棉区种植密度、植株生长阶段、光照、温度、湿度等环境因素的统一；3)开花期结束后，沿转基因棉花区至常规棉区的线性方向，在每条常规棉区中等距离取F1代样本检测是否存在外源基因，确定不同隔离方式对基因漂移频率、距离的影响。
申请号：CN201610630140.6
专利号：CN106233975-A
公开/公告号：CN106233975-A
申请/专利权人：中国农业大学
发明/设计人：刘小侠;董杰;张博宇;李贞;张青文
分类号：A01G1/00
主分类号：A01G1/00
法律状态：实质审查
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