中文摘要：本发明提供一种用于转基因玉米检测的探针组合、液相芯片、试剂盒及其应用，涉及转基因检测技术领域。探针组合包括由根据转基因玉米中常见的外源基因Pat、Bar、Cry1Ab、Cry1Ac、Cry105、EPSPS?P0、EPSPS?P2、P35S、T35S以及Nos?3’设计出10种检测探针和根据玉米内源基因Ivr1设计的阳性探针，该探针组合分别与荧光编码微球偶联后，可用于检测出转基因玉米是否含有上述转基因成分，其具有操作简单、特异性强、灵敏度高、通量大的特点，且其对种子站、农科院所和边境口岸对转基因玉米检测具有重要作用。
外文摘要：NOVELTY - Probe combination comprises 5' end labeled C12 molecular arm with amino-modified positive probes and detection probes. The positive probe comprises a 59 base pair sequence (SEQ ID NO: 33) fully defined in the specification, and detection probe comprises one of (10) 48-59 base pair sequences (SEQ ID NOs: 23-32) all fully defined in the specification. The positive probe and detection probe are reverse complementary sequence.
USE - The probe combination, or kit is useful for detecting transgenic corn, or transgenic corn components (all claimed).
ADVANTAGE - The probe combination enables to detect transgenic corn components with high specificity, and sensitivity in a simple manner.
DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following:
(1) liquid phase chip, comprising probe combination with hydroxylated fluorescently encoded microspheres;
(2) kit, comprising liquid phase chip, control group and detection group, where detection group comprises at least one of 1-10 detection groups, and control group comprises forward primer, reverse primer and positive probe with C12 molecular arms and amino-modified markers, detection primer comprises forward primer, and reverse primer and 5' end labeled with C12 molecular arms and amino-modified marker detection probe, forward primer of control group, detection group and reverse primers are labeled with a biotin at 5' end, forward primer of 1-10 detection group and control group comprises one of (11) 20-31 base pair sequences (SEQ ID NOs: 1-11) all fully defined in the specification, reverse primer of detection group and control group comprises one of (11) 20-25 base pair sequences (SEQ ID NOs: 12-22) all fully defined in the specification, and detection probes of 1-10 detection group and control group comprises one of (11) 48-59 base pair sequences (SEQ ID NOs: 23-33) all fully defined in ! the spec ification; and
(3) use of kit for detecting transgenic corn components.
tgatatggccgcggtttgtgat (SEQ ID NO: 1), ggggatctaccatgagccca (SEQ ID NO: 2), cgacatctccttgtccttgac (SEQ ID NO: 3), gttagactcaacagcagtgga (SEQ ID NO: 4), actcgatcaggtacaatgcca (SEQ ID NO: 5), gcgcgatcatacggaaaagat (SEQ ID NO: 6), tcgtgaccacactgaaaagat (SEQ ID NO: 7), aagacgttccaaccacgtcttcaa (SEQ ID NO: 8), ggcccagcgtaagcaatacc (SEQ ID NO: 12), ggctcggtacggaagttgac (SEQ ID NO: 13), ctcaatttgcaccaggaatgc (SEQ ID NO: 14), gagaagatggatgaattacccca (SEQ ID NO: 15), gcatctgttaggctctccac (SEQ ID NO: 16), tcgatgacttggccggtgag (SEQ ID NO: 17), and tcaatcacttgaccggtgag (SEQ ID NO: 18).
主权项：一种用于转基因玉米检测的探针组合，其特征在于，其包括5’端标记C12分子臂和氨基修饰的阳性探针和检测探针，所述阳性探针的碱基序列为SEQ?ID?NO.33所示，且所述检测探针的碱基序列为选自SEQ?ID?NO.23?SEQ?ID?NO.32所示序列的中的任一种；或所述阳性探针的碱基序列为SEQ?ID?NO.33所示序列的反向互补序列，所述检测探针的碱基序列为选自SEQ?ID?NO.23?SEQ?ID?NO.32所示序列的中的任一种的反向互补序列。
申请号：CN201610920668.7
专利号：CN106498049-A
公开/公告号：CN106498049A
申请日：2016-10-21
公开/公告日：2017-03-15
申请/专利权人：上海泽衡生物科技有限公司
发明/设计人：郭尚 ; 秦子苏 ; 王岳华 ; 周均
分类号：C12Q1/68 ; C12N15/11
主分类号：C12Q1/68
法律状态：实质审查
点击下载：一种用于转基因玉米检测的探针组合、液相芯片、试剂盒及其应用