中文摘要：本发明公开了一种快速同步检测商业化应用转基因棉花的多重PCR检测方法，所述引物具有较强的特异性，能够用于多重PCR检测分析。所述检测方法提供了一种检测快速、操作简便、扩增性能好、灵敏度高的转基因检测方法，实现了转基因棉花多个转化体同步检测。利用毛细管电泳对PCR扩增产物进行分析，其片段大小区分率可达数个碱基，分辨率高。本发明的引物和检测方法为转基因棉花提供了一种快速、有效、可靠的检测方法。
外文摘要：NOVELTY - A multiplex PCR primer for rapid simultaneous detection of 6 commercialized transgenic cotton, is new. The primer sequences comprise MON1445-F, MON1445-R, GHB614-F, GHB614-R, MON15985-F, MON15985-R, MON88913-F, MON88913-R, LLcotton25-F, LLcotton25-R, MON531-F and MON531-R.
USE - The multiplex PCR primer is useful for rapid simultaneous detection of 6 commercialized transgenic cotton (claimed).
ADVANTAGE - The multiplex PCR primer detects commercialized transgenic cotton rapidly.
DETAILED DESCRIPTION - A multiplex PCR primer for rapid simultaneous detection of 6 commercialized transgenic cotton, is new. The primer sequences comprise MON1445-F comprising a base pair sequence of: 5'-aatgctggattttctgcctgtg-3' (SEQ ID NO: Not defined), MON1445-R comprising a base pair sequence of: 5'-tccaaaagtcatgcatcatttctca-3' (SEQ ID NO: Not defined), GHB614-F comprising a base pair sequence of: 5'-caaatacacttggaacgacttcgt-3' (SEQ ID NO: Not defined), GHB614-R comprising a base pair sequence of: 5'-gcaggcatgcaagcttttaaa-3' (SEQ ID NO: Not defined), MON15985-F comprising a base pair sequence of: 5'-atttgatgcactatgtcttcgtcta-3' (SEQ ID NO: Not defined), MON15985-R comprising a base pair sequence of: 5'-caatggaatccgaggaggt-3' (SEQ ID NO: Not defined), MON88913-F comprising a base pair sequence of: 5'-taataacgctgcggacatctac-3' (SEQ ID NO: Not defined), MON88913-R comprising a base pair sequence of: 5'-gagaagcgagacctacaagcc-3' (SEQ ID NO: Not defined), LLcotton25-F comprising a base pair sequence of: 5'-caaggaactattcaactgag-3' (SEQ ID NO: Not defined), LLcotton25-R comprising a base pair sequence of: 5'-caacctgtctgtttgctgac-3' (SEQ ID NO: Not defined), MON531-F comprising a base pair sequence of: 5'-aagagaaaccccaatcataaaa-3' and MON531-R comprising a base pair sequence of: 5'-gagaatgcggtaaagatacgtc-3' (SEQ ID NO: Not defined). An INDEPENDENT CLAIM is included for method for rapid simultaneous detection of 6 commercial PCR applications o! f transg enic cotton, which involves synthesizing the primers, preparing diluted to-be-measured DNA, preparing PCR reaction system, and subjecting the PCR reaction product for capillary electrophoresis.
主权项：一种快速同步检测6种商业化应用转基因棉花的多重PCR引物，其特征在于，包括以下引物序列：MON1445?F：5'?AATGCTGGATTTTCTGCCTGTG?3'；MON1445?R：5'?TCCAAAAGTCATGCATCATTTCTCA?3'；GHB614?F：5'?CAAATACACTTGGAACGACTTCGT?3'；GHB614?R：5'?GCAGGCATGCAAGCTTTTAAA?3'；MON15985?F：5'?ATTTGATGCACTATGTCTTCGTCTA?3'；MON15985?R：5'?CAATGGAATCCGAGGAGGT?3'；MON88913?F：5'?TAATAACGCTGCGGACATCTAC?3'；MON88913?R：5'?GAGAAGCGAGACCTACAAGCC?3'；LLcotton25?F：5'?CAAGGAACTATTCAACTGAG?3'；LLcotton25?R：5'?CAACCTGTCTGTTTGCTGAC?3'；MON531?F：5'?AAGAGAAACCCCAATCATAAAA?3'；MON531?R：5'?GAGAATGCGGTAAAGATACGTC?3'。
申请号：CN201610932231.5
专利号：CN106434946-A
公开/公告号：CN106434946A
申请日：2016-10-31
公开/公告日：2017-02-22
申请/专利权人：四川民族学院
发明/设计人：李忆
分类号：C12Q1/68 ; C12N15/11
主分类号：C12Q1/68
法律状态：实质审查
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