中文摘要：一种转基因青蒿的环境安全评价方法，该方法对转基因青蒿的农艺性状、抗逆性以及基因漂移进行测试，同时以野生型受体青蒿作对照，评估中设置的参数涵盖了转基因青蒿和其野生型受体差异性的各指标，为转基因青蒿的环境释放建立了模型，在说明两者实质等同性上具有重要示范意义，本发明从以上三个方面建立的评价体系操作简单，易于实现，直观性强，结果准确性高，该方法对转基因青蒿的环境安全评价提供了待测指标，为转基因青蒿品种的商业化风险评估提供了技术支撑。
外文摘要：NOVELTY - Transgenic Artemisia carvifolia environmental safety assessment comprises e.g. (i) transplanting the transgenic Artemisia carvifolia plant agronomic morphological measurement from greenhouse to field planting area, transplanting for 3-4 months, measuring plant morphology parameter, comparing to wild type Artemisia carvifolia, (ii) evaluating resistance in the greenhouse for transgenic Artemisia carvifolia of salt stress, drought stress, herbicide tolerance and winter ability evaluation, and comparing to the wild type Artemisia carvifolia.
USE - The method is useful for transgenic Artemisia carvifolia environmental safety assessment (claimed).
ADVANTAGE - The method is simple, provides the index and provides the technical support for commercial risk assessment of transgenic Artemisia carvifolia variety.
DETAILED DESCRIPTION - Transgenic Artemisia carvifolia environmental safety assessment comprises (i) transplanting the transgenic Artemisia carvifolia plant agronomic morphological measurement from greenhouse to field planting area, transplanting for 3-4 months, measuring plant morphology parameter, comparing to wild type Artemisia carvifolia, (ii) evaluating resistance in the greenhouse for transgenic Artemisia carvifolia of salt stress, drought stress, herbicide tolerance and winter ability evaluation, comparing to the wild type Artemisia carvifolia, (iii) measuring gene drift set rectangular test field to drift the gene test, where the area not less than 5mu, transgenic Artemisia carvifolia containing exogenous gene is planted in the center and 4-7 concentric circles, increasing the radius of the concentric circle from inside to outside by 0.8-1.4 m. a rectangular experimental field of the shortest side length is 4-9 times of the maximum radius of the concentric circles, concentric outer planting Artemisia carvifolia wild type, providing the experimental field is ! around t he rectangular physical isolation region in the wild type receptor Artemisia carvifolia planting area is set not less than 72 test points along more than 8 directions are concentric circles, harvesting the Artemisia carvifolia seed of each test point, measuring germination percentage of seed, and kanamycin resistance screening plant for card kanamycin resistance using PCR method to further verify whether it contains the exogenous gene inserting.
 
主权项：一种转基因青蒿的环境安全评价方法，包括以下步骤：1)农艺性状形态学测定将转基因青蒿植株从温室内移栽至大田种植区域内，移植3?4个月后，测定植株的形态学参数，同时，以野生型青蒿作对照；2)抗逆性评价在温室中进行转基因青蒿的盐胁迫、干旱胁迫、除草剂耐受性以及越冬能力评价，同时，以野生型青蒿作对照；3)基因漂移测定设置矩形实验场来进行基因漂移试验，其面积不低于5亩，含外源基因的转基因青蒿种植于中心点及其4?7个同心圆中，同心圆的半径从内到外以0.8?1.4m逐个增加，矩形实验场的最短边长应是同心圆最大半径的4?9倍，同心圆外种植野生型青蒿，同时，在该矩形实验场周围设置物理隔离区；在野生型受体青蒿种植区内，沿同心圆的8个以上方向设置不少于72个测试点，呈米字形，收获各测试点的青蒿种子，测定种子发芽率、并进行卡那
 
申请号：CN201611037837.9
专利号：CN106718179-A
公开/公告号：CN106718179-A
申请日：2016-11-23
公开/公告日：2017-05-31
申请/专利权人：上海市农业科学院
发明/设计人：刘华 ; 唐雪明 ; 王金斌 ; 白蓝 ; 蒋玮 ; 吴潇 ; 潘爱虎 ; 贾军伟
分类号：A01G7/00 ; A01G7/06 ; C12Q1/68
主分类号：A01G7/00
法律状态：实质审查
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