中文摘要：本发明具体公开了一种粮油中转基因成分的快速检测装置，包括加样装置、多根中空纤维素聚丙烯膜和多孔板体，所述中空纤维聚丙烯膜上附着有与总蛋白中的转基因蛋白质特异性结合的一抗，使该转基因蛋白质与所述一抗结合并附着到所述中空纤维聚丙烯膜上。本发明设计简单，使用方便，易于操作。本发明创造性地将转基因表达蛋白的抗体固定在纤维聚丙烯膜，从而通过使待检测原材料的总蛋白流过该检测装置来检测原材料中是否存在转基因蛋白质，能够有效快速地检测是否存在转基因蛋白。本发明同时设置两个加样孔，两组中空纤维聚丙烯膜，因而本发明的装置可以同时进行两组样品检测。
外文摘要：NOVELTY - Device comprises a sampling device, multiple hollow cellulose polypropylene membrane and porous plate (P1). The hollow cellulose polypropylene membrane is attached with a primary antibody that specifically binds to the transgenic protein in the total protein. The sampling device comprises porous plate (P2). The porous plate (P2) is provided with cone having a cavity. The cone diameter gradually reduced from bottom to top.
USE - The device is useful for rapidly detecting genetically modified components or proteins in grains and oil.
ADVANTAGE - The device is simple in design, easy to use and operate and simultaneously detects two sets of samples.
DETAILED DESCRIPTION - Device comprises a sampling device, multiple hollow cellulose polypropylene membrane and porous plate (P1). The hollow cellulose polypropylene membrane is attached with a primary antibody that specifically binds to the transgenic protein in the total protein. The sampling device comprises porous plate (P2). The porous plate (P2) is provided with cone having a cavity. The cone diameter gradually reduced from bottom to top. The one end of the multiple hollow cellulose polypropylene membrane fixed on the porous plate (P2) and connected with the hole of the porous plate (P2) and the other end of the multiple hollow cellulose polypropylene membrane fixed to the porous plate (P1) and connected with a hole in the porous plate (P1), where when the total protein passes through the detection device, then sealing the hole on the porous plate (P1) to make the flow of total protein occurs only between the component with hollow cellulose polypropylene membrane having one end and a film hole of the hollow cellulose polypropylene membrane, the transgenic protein is combined with the first antibody and attached to the hollow cellulose polypropylene membrane.
主权项：一种粮油中转基因成分的快速检测装置，其特征在于，包括加样装置、多根中空纤维素聚丙烯膜和多孔板体，所述中空纤维聚丙烯膜上附着有与总蛋白中的转基因蛋白质特异性结合的一抗，所述加样装置包括第2多孔板体，第2多孔板体上设置有与其形成一空腔的圆锥体，所述圆锥体直径由下到上逐渐减小，所述多根中空纤维聚丙烯膜的一端固定在所述第2多孔板体且与所述第2多孔板体上的孔洞连通，所述多根中空纤维聚丙烯膜的另一端固定在所述第1多孔板体且与所述第1多孔板体上的孔洞连通，当总蛋白通过所述检测装置时，封闭所述第1多孔板体上的孔洞，以使总蛋白的流动仅发生在所述多根中空纤维聚丙烯膜的一端和所述中空纤维聚丙烯膜的膜孔之间，使该转基因蛋白质与所述一抗结合并附着到所述中空纤维聚丙烯膜上。
申请号：CN201710064520.2
专利号：CN106645702-A
公开/公告号：CN106645702-A
申请日：2017-02-04
公开/公告日：2017-05-10
申请/专利权人：上海为然环保科技有限公司
发明/设计人：不公告发明人
分类号：G01N33/544
主分类号：G01N33/544
法律状态：实质审查
点击下载：一种粮油中转基因成分的快速检测装置