中文摘要：本发明提供一种水稻MSP1基因突变体及其应用，属于基因工程技术领域。本发明将籼稻品种93?11经钴60辐射诱变引起水稻MSP1基因编码区第1879个碱基的1个T碱基替换为1个C碱基，将该MSP1基因突变体命名为msp1?1972，其核苷酸序列如SEQ?ID?No.1所示，进一步证实该突变体引起水稻隐性雄性核不育，可用于制备隐性雄性核不育的转基因水稻，在水稻种质资源的遗传改良育种中作用重大。本发明还提供了该突变体的分子标记鉴定方法及其在育种制种中的应用。
外文摘要：NOVELTY - Rice MSP1 mutant gene msp1-1972 is new, in which rice MSP1 gene nucleotide t at position 1879 is replaced by c and mutation site is located on exon.
USE - Rice MSP1 mutant gene msp1-1972 used for preparing transgenic plant or transgenic rice with recessive male sterility (claimed).
ADVANTAGE - The mutant gene msp1-1972 improves rice cultivation and seed production.
DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for:
(1) expression vector comprising rice MSP1 mutant gene msp1-1972;
(2) host cell containing expression vector;
(3) molecular marker for detecting rice MSP1 mutant gene msp1-1972, amplified by primer pairs comprising upstream primer 1972-F comprising attttgtcttccaaccagcgg, and downstream primer 1972-R comprising accatcaccattgcacagt; and
(4) molecular labeling of rice MSP1 mutant gene msp1-1972 by amplifying to-be-detected plant genomic DNA by primer pairs, cleaving amplified product by HaeIII enzyme at 37 degrees C, and detecting cleaved product, in which if the cleaved product is 20 bp shorter than wild type 93-11 cleaved product, this indicates presence of rice MSP1 mutant gene msp1-1972.
主权项：一种水稻MSP1基因突变体msp1?1972，其为水稻MSP1基因第1879位碱基的T替换为C，该突变位点位于外显子上。
申请号：CN201610714718.6
专利号：CN106434708-A
公开/公告号：CN106434708-A
申请日：2016-08-24
公开/公告日：2017-02-22
申请/专利权人：海南波莲水稻基因科技有限公司
发明/设计人：黄培劲 ; 李新鹏 ; 李京琳 ; 张维 ; 安保光 ; 龙湍
分类号：C12N15/54 ; C12N9/12 ; C12N15/11 ; C12Q1/68 ; A01H5/00
主分类号：C12N15/54
法律状态：实质审查
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