中文摘要：本发明属于生物技术领域，涉及基因的定量分析方法，具体是指含有gat?tpin?Ⅱ结构转基因植物实时PCR精准检测方法。本发明采用设计的特异性上游引物序列、下游引物序列、荧光探针序列、DNA稀释液以及Taqman?Master?mix(2×)和水配制成PCR反应体系，进行定量PCR检测。本发明主要建立了一种高扩增效率、高准确度的Taqman定量PCR检测技术，适用于国内农业转基因生物及产品监督检验、进出境口岸转基因生物及产品检验、企业内部进口原材料含gat?tpin?Ⅱ结构的生物成分检测。
外文摘要：NOVELTY - Primer and probe comprise upstream primer which comprises nucleotide sequence of 25 nucleobases (SEQ ID NO: 1) and downstream primer comprises nucleotide sequence of 23 nucleobases (SEQ ID NO: 2) and fluorescent probe sequence comprises nucleotide sequence of 30 nucleobases (SEQ ID NO: 3).
USE - Primer and probe used for detecting real-time PCR containing a gat-tpinII transgenic plant (claimed).
ADVANTAGE - The primer and probe are utilized for detecting real-time PCR containing a gat-tpinII transgenic plant with high amplification efficiency and high accuracy of PCR detection technology.
DETAILED DESCRIPTION - Primer and probe comprise upstream primer which comprises nucleotide sequence of 25 nucleobases (SEQ ID NO: 1), i.e. agttrggmttcagygagcarggaga, and downstream primer comprises nucleotide sequence of 23 nucleobases (SEQ ID NO: 2); i.e ccaatccagaagatggacaagtc and fluorescent probe sequence comprises nucleotide sequence of 30 nucleobases (SEQ ID NO: 3); i.e cckccagtwggacctcacatcctgatgtat. An INDEPENDENT CLAIM is included for a method for detecting real-time PCR containing a gat-tpinII transgenic plant, which involves:
(A) synthesizing the upstream primers and the fluorescent probe used in combination with the primer;
(B) preparing DNA dilution;
(C) preparing DNA dilution in the downstream primer sequence of gat-tpin II; and
(D) preparing and detecting PCR reaction system quantitatively.
 
主权项：含有gat?tpinⅡ结构转基因植物实时PCR精准检测的引物和探针，其特征在于,上游引物序列，gat?tpinⅡ?F1：5'?AGTTRGGMTTCAGYGAGCARGGAGA?3'；下游引物序列，gat?tpinⅡ?R：5'?CCAATCCAGAAGATGGACAAGTC?3'；荧光探针序列，gat?tpinⅡ?P2：5'?FAM?CCKCCAGTWGGACCTCACATCCTGATGTAT?TAMRA?3'。
申请号：CN201710104685.8
专利号：CN106701999-A
公开/公告号：CN106701999-A
申请日：2017-02-24
公开/公告日：2017-05-24
申请/专利权人：四川省农业科学院分析测试中心
发明/设计人：张富丽 ; 常丽娟 ; 尹全 ; 宋君 ; 王东 ; 郭灵安 ; 雷绍荣
分类号：C12Q1/68 ; C12N15/11
主分类号：C12Q1/68
法律状态：实质审查
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