中文摘要：本发明公开了一种Cry1Ie单克隆抗体细胞株，于2016年5月19日，保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏编号为CGMCC NO.12297。本发明通过对Cry1Ie蛋白的氨基酸序列的抗原性、亲水性和表位性分析，选定抗原表位，人工合成特异性肽段，进而得到抗Cry1Ie蛋白的单克隆杂交瘤细胞株，为研制灵敏、快速的Bt Cry1Ie晶体蛋白检测试剂盒(试纸条)奠定了基础，在Cry1Ie蛋白定性、定量检测方面具有广阔的前景。本发明的单克隆抗体，对在植物中表达的Bt Cry1Ie蛋白具有高亲和力，用于定性或定量检测棉花、玉米等转基因植物中的Bt Cry1Ie蛋白，具有特异性强、快速、灵敏的优点。
外文摘要：NOVELTY - Cry1Ie monoclonal antibody cell strain (CGMCC NO.12297), is new.
USE - The Cry1Ie monoclonal antibody cell strain and monoclonal antibody is useful for detecting Cry1Ie protein (claimed) expression in a plant and qualitative or quantitative quantity detection of Cry1Ie protein in transgenic plants, preferably cotton and corn.
ADVANTAGE - The Cry1Ie monoclonal antibody cell strain has strong specificity; and is rapid and sensitive.
DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for:
 (1) monoclonal antibody, produced by Cry1Ie monoclonal antibody cell strain, where the monoclonal antibody is a monoclonal antibody that is specific for the Cry1Ie protein;
 (2) use of Cry1Ie monoclonal antibody cell strain and monoclonal antibody for detecting Cry1Ie protein;
 (3) Cry1Ie protein detection test paper comprising monoclonal antibodies secreted by Cry1Ie monoclonal antibody cell strain; and
 (4) Cry1Ie protein detection reagent box comprising monoclonal antibodies secreted by Cry1Ie monoclonal antibody cell strain.
 
主权项：一种Cry1Ie单克隆抗体细胞株，于2016年5月19日，保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏编号为CGMCC NO.12297。
 
申请号：CN201610816083.0
专利号：CN106834234-A
公开/公告号：CN106834234A
申请/专利权人：中国农业科学院植物保护研究所
发明/设计人：何康来; 徐丽娜; 王振营; 王月琴; 孙丹丹
分类号：C12N5/20; C07K16/12; G01N33/68; G01N33/577; C12R1/91;
主分类号：C12N5/20
法律状态：实质审查
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