中文摘要：本发明公开了一种应用RPA技术对转基因玉米BT176品系特异性鉴定的方法。该方法首先提取待测样品的DNA作为模板，利用筛选的引物及探针进行RPA快速扩增及实时荧光检测，如果得到扩增曲线，则证明所检样品含有转基因玉米BT176成分。本发明根据外援插入DNA序列与玉米基因组的连接区域设计大量RPA引物，从中筛选出一对可快速有效检测出转基因玉米BT176成分的引物及探针组合，首次提供了转基因玉米BT176品系特异性的RPA检测方法。
外文摘要：NOVELTY - Primer and probe comprising a nucleotide sequence of the forward primer having 35 base pair sequence (SEQ ID NO: 1) fully defined in the specification, the nucleotide sequence of the reverse primer having 35 base pair sequence (SEQ ID NO: 2) fully defined in the specification, and the nucleotide sequence of the probe having 37 base pair sequence (SEQ ID NO: 3) fully defined in the specification, are new.
USE - The primer and probe or kit is useful for specific identification of transgenic maize BT176 strain using RPA technology (all claimed).
ADVANTAGE - The primer and probe rapidly detects BT176 strain of transgenic maize.
DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following:
(1) kit comprising the above-mentioned primer and probe; and
(2) method for specific identification of transgenic maize BT176 strain using recombinase polymerase amplification (RPA) technology involves extracting the DNA of the sample to be detected as template, performing RPA rapid amplification and real-time fluorescence detection using the primer and probe, if the amplification curve is obtained, it is proved that the detected sample contains transgenic maize BT176 component, if the amplification curve is not available, it is proved that the sample does not contain transgenic maize BT176 component.
主权项：一种应用RPA技术对转基因玉米BT176品系特异性鉴定的引物和探针，其特征在于，所述正向引物的核苷酸序列如SEQ ID NO：1所示，反向引物的核苷酸序列如SEQ ID NO：2所示，探针的核苷酸序列如SEQ ID NO：3所示。
申请号：CN201710047608.3
专利号：CN106801092-A
公开/公告号：CN106801092A
申请/专利权人：中国农业科学院生物技术研究所
发明/设计人：宛煜嵩; 李凯; 李亮; 董美; 金芜军; 刘卫晓; 温洪涛; 潘海会; 王永; 高进; 王迪
分类号：C12Q1/68; C12N15/11;
主分类号：C12Q1/68
法律状态：实质审查
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