中文摘要：本发明提供一种玉米SILKY1基因突变体及其应用，属于基因工程技术领域。本发明将杂交玉米品种京科糯2000经钴60辐射诱变引起玉米SILKY1基因编码区第239个碱基后插入两个碱基GA，将该SILKY1基因突变体命名为silky1‑3552，其核苷酸序列如SEQ ID No.1所示，进一步证实该突变体引起玉米隐性雄性核不育，可用于制备玉米隐性雄性核不育系，在玉米种质资源的遗传改良育种中作用重大。本发明还提供了该突变体的分子标记鉴定方法及其在育种制种中的应用。
外文摘要：USE - Maize SILKY1 gene mutant silky1-3552 for preparing transgenic plant, preparing transgenic rice with recessive genetic male sterility, and improving breeding and seed production of maize (all claimed).
DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for:
(1) an expression vector comprises SILKY1 gene mutant silky1-3552;
(2) host cell comprises expression vector;
(3) molecular marker for detecting maize SILKY1 gene mutant silky1-3552, and for the preparation of transgenic maize with recessive genetic male sterility, is amplified by the primer pair having upstream primer containing nucleobase sequence of 21 nucleobases (SEQ ID NO: 2), which are cgtgtgtgttggttggttggt, and downstream primer containing nucleobase sequence of 22 nucleobases (SEQ ID NO: 3), which are gacggacctcatactgctcgat; and
(4) a method for detecting maize SILKY1 gene mutant silky1-3552, which involves amplifying genomic DNA of the plant to be detected by using the primer pair, and then detecting amplification product where if the primer pair is able to amplify a fragment of 2 basepair longer than that of the wild-type rice-based amplification product, then maize SILKY1 gene mutant silky1-3552 is present in the plant to be tested.
主权项：一种玉米SILKY1基因突变体silky1-3552，其为玉米SILKY1基因在编码区第239个碱基后插入两个碱基GA，该突变位点位于第二外显子上。
申请号：CN201710199138.2
专利号：CN106754975-A
公开/公告号：CN106754975A
申请/专利权人：海南波莲水稻基因科技有限公司
发明/设计人：黄培劲; 李新鹏; 李京琳; 张维; 安保光; 龙湍
分类号：C12N15/29; C12N15/82; C12N15/11; C12Q1/68; A01H5/00;
主分类号：C12N15/29
法律状态：实质审查
点击下载：一种玉米SILKY1基因突变体及其分子鉴定方法和应用