中文摘要：本发明涉及一种转基因玉米事件GT1外源插入片段旁侧序列及其应用。本发明提供的富含α-生育酚的转基因玉米事件GT1经鉴定有一个插入位点，这个插入位点的外源插入片段的3’端旁侧序列如序列1所示。本发明提供了用于检测该旁侧序列的引物。本发明的转基因玉米事件GT1外源插入片段旁侧序列的鉴定适用于对富含α-生育酚的转基因玉米GT1(包括亲本、杂种F1和后代)及其制品(包括植株、组织、种子及其制品)的检测。
外文摘要：NOVELTY - alpha -tocopherol transgenic corn event GT1 insertion site exogenous insert of 3' end flanking DNA comprising nucleotide shown as SEQ ID NO:1 (sequence not defined), is new.
USE - The flanking DNA and specific primer are useful for detection of transgenic corn (claimed).
ADVANTAGE - The flanking DNA: is suitable for detection of transgenic maize GT1 rich in alpha -tocopherol (including parent, hybrid F1 and offspring) and its products (including plants, tissues, seeds and their products).
DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for
(1) specific primers for detecting the DNA, comprising nucleotide sequence as Nos forward primer: 5'-atgattagagtcccgcaattatac-3',and GT1 reverse primer: 5'-gggatctgtcgatcgacaag-3'; and
(2) detecting transgenic corn event GT1, comprising carrying out PCR reaction by using the primers, using total DNA of the sample as a template, and determining results according to the electrophoretic fragment of the PCR product, if 438 bp fragment is amplified, then the result is positive.
主权项：α-生育酚转基因玉米事件GT1插入位点的外源插入片段的3’端旁侧DNA，其核苷酸如序列1所示。 
申请号：CN201610005926.9
专利号：CN106939308-A
公开/公告号：CN106939308A
申请/专利权人：深圳市农科集团有限公司; 中国农业科学院生物技术研究所;
发明/设计人：王磊; 赵杨敏; 张兰; 郑平; 徐妙云; 林玉群; 范云六; 金英子
分类号：C12N15/11; C12Q1/68;
主分类号：C12N15/11
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